4l55

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X-ray structure of the adduct between bovine pancreatic ribonuclease and AziRu

Structural highlights

4l55 is a 2 chain structure with sequence from Bos taurus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.65Å
Ligands:RU
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RNAS1_BOVIN Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA.[1]

Publication Abstract from PubMed

A Raman-assisted crystallographic study on the adduct between AziRu, a Ru(III) complex with high antiproliferative activity, and RNase A is presented. The protein structure is not perturbed significantly by the Ru label. The metal coordinates to ND atoms of His105 or of His119 imidazole rings, losing all of its original ligands but retaining octahedral, although distorted, coordination geometry. The AziRu binding inactivates the enzyme, suggesting that its antitumor action can be exerted by a mechanism of competitive inhibition.

Investigating the ruthenium metalation of proteins: X-ray structure and Raman microspectroscopy of the complex between RNase A and AziRu.,Vergara A, Russo Krauss I, Montesarchio D, Paduano L, Merlino A Inorg Chem. 2013 Oct 7;52(19):10714-6. doi: 10.1021/ic401494v. Epub 2013 Sep 16. PMID:24093479[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
1 reviews cite this structure
Anticancer Ruthenium(III) Complexes and Ru(III)-Containing Nanoformulations: An Update on the Mechanism of Action and Biological Activity.
Riccardi et al. (2019)
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18 other articles
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See Also

References

  1. ↑ delCardayre SB, Ribo M, Yokel EM, Quirk DJ, Rutter WJ, Raines RT. Engineering ribonuclease A: production, purification and characterization of wild-type enzyme and mutants at Gln11. Protein Eng. 1995 Mar;8(3):261-73. PMID:7479688
  2. ↑ Vergara A, Russo Krauss I, Montesarchio D, Paduano L, Merlino A. Investigating the ruthenium metalation of proteins: X-ray structure and Raman microspectroscopy of the complex between RNase A and AziRu. Inorg Chem. 2013 Oct 7;52(19):10714-6. doi: 10.1021/ic401494v. Epub 2013 Sep 16. PMID:24093479 doi:http://dx.doi.org/10.1021/ic401494v

Contents


PDB ID 4l55

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