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4lnq

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Crystal structure of Ifi202 HINa domain in complex with 20bp dsDNA

Structural highlights

4lnq is a 4 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

IFI2_MOUSE Inhibits the transcriptional activity of several transcription factors, including NF-kappa-B p50 and p65, FOS, JUN, E2F1, E2F4, MYOD1 and myogenin. Has anti-apoptotic effects due to inhibition of the transcriptional activity of p53. Binds dsDNA in the cytosol. Is involved in innate immune response and has anti-inflammatory activity. Inhibits caspase activation in response to cytosolic DNA and may inhibit the activation of the AIM2 inflammasome, probably via association with AIM2.^[1] ^[2]

Publication Abstract from PubMed

The HIN-200 family of proteins play significant roles in inflammation-related processes. Among them, AIM2 (absent in melanoma 2) and IFI16 (gamma-interferon-inducible protein 16) recognize double-stranded DNA to initiate inflammatory responses. In contrast, p202, a mouse interferon-inducible protein containing two HIN domains (HINa and HINb), has been reported to inhibit Aim2-mediated inflammatory signalling in mouse. To understand the inhibitory mechanism, the crystal structure of the p202 HINa domain in complex with a 20 bp DNA was determined, in which p202 HINa nonspecifically recognizes both strands of DNA through electrostatic attraction. The p202 HINa domain binds DNA more tightly than does AIM2 HIN, and the DNA-binding mode of p202 HINa is different from that of the AIM2 HIN and IFI16 HINb domains. These results, together with the reported data on p202 HINb, lead to an interaction model for full-length p202 and dsDNA which provides a conceivable mechanism for the negative regulation of Aim2 inflammasome activation by p202.

Structural mechanism of DNA recognition by the p202 HINa domain: insights into the inhibition of Aim2-mediated inflammatory signalling.,Li H, Wang J, Wang J, Cao LS, Wang ZX, Wu JW Acta Crystallogr F Struct Biol Commun. 2014 Jan;70(Pt 1):21-9. doi:, 10.1107/S2053230X1303135X. Epub 2013 Dec 24. PMID:24419611^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Xin H, D'Souza S, Jorgensen TN, Vaughan AT, Lengyel P, Kotzin BL, Choubey D. Increased expression of Ifi202, an IFN-activatable gene, in B6.Nba2 lupus susceptible mice inhibits p53-mediated apoptosis. J Immunol. 2006 May 15;176(10):5863-70. PMID:16670293
↑ Roberts TL, Idris A, Dunn JA, Kelly GM, Burnton CM, Hodgson S, Hardy LL, Garceau V, Sweet MJ, Ross IL, Hume DA, Stacey KJ. HIN-200 proteins regulate caspase activation in response to foreign cytoplasmic DNA. Science. 2009 Feb 20;323(5917):1057-60. doi: 10.1126/science.1169841. Epub 2009, Jan 8. PMID:19131592 doi:10.1126/science.1169841
↑ Li H, Wang J, Wang J, Cao LS, Wang ZX, Wu JW. Structural mechanism of DNA recognition by the p202 HINa domain: insights into the inhibition of Aim2-mediated inflammatory signalling. Acta Crystallogr F Struct Biol Commun. 2014 Jan;70(Pt 1):21-9. doi:, 10.1107/S2053230X1303135X. Epub 2013 Dec 24. PMID:24419611 doi:http://dx.doi.org/10.1107/S2053230X1303135X