4wt8

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Crystal Structure of bactobolin A bound to 70S ribosome-tRNA complex

Structural highlights

4wt8 is a 18 chain structure with sequence from Thermus thermophilus HB8. This structure supersedes the now removed PDB entries 4wus, 4wwe and 4wwt. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:3V6, MG, PAR
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RS2_THET8 Spans the head-body hinge region of the 30S subunit. Is loosely associated with the 30S subunit.[HAMAP-Rule:MF_00291_B]

Publication Abstract from PubMed

The ribosome is the target of a large number of antibiotics. Here, we report a 3.4-A-resolution crystal structure of bactobolin A bound to 70S ribosome-tRNA complex. The antibiotic binds at a previously unseen site in the 50S subunit and displaces tRNA bound at the P-site. It thus likely has a similar mechanism of action as blasticidin S despite binding to a different site. The structure also rationalizes previously identified resistance mutations.

Bactobolin A Binds to a Site on the 70S Ribosome Distinct from Previously Seen Antibiotics.,Amunts A, Fiedorczuk K, Truong TT, Chandler J, Peter Greenberg E, Ramakrishnan V J Mol Biol. 2015 Jan 3. pii: S0022-2836(14)00654-8. doi:, 10.1016/j.jmb.2014.12.018. PMID:25562208[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
9 reviews cite this structure
Bacterial Protein Synthesis as a Target for Antibiotic Inhibition.
Arenz et al. (2016)
8 more
17 other articles
No citations found

See Also

References

  1. ↑ Amunts A, Fiedorczuk K, Truong TT, Chandler J, Peter Greenberg E, Ramakrishnan V. Bactobolin A Binds to a Site on the 70S Ribosome Distinct from Previously Seen Antibiotics. J Mol Biol. 2015 Jan 3. pii: S0022-2836(14)00654-8. doi:, 10.1016/j.jmb.2014.12.018. PMID:25562208 doi:http://dx.doi.org/10.1016/j.jmb.2014.12.018

Contents


PDB ID 4wt8

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