4xqj

From Proteopedia

Jump to: navigation, search

Crystal structure of AgrA LytTR domain in complex with promoters

Structural highlights

4xqj is a 6 chain structure with sequence from Staphylococcus aureus subsp. aureus COL and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.9Å
Ligands:CA, EDO, EOH
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

AGRA_STAAC Required for high-level post-exponential phase expression of a series of secreted proteins.

Publication Abstract from PubMed

The intracellular trigger for the quorum sensing response mechanism in Staphylococcus aureus involves the phosphorylation of the response regulator AgrA by the membrane anchored histidine kinase AgrC. AgrA activates transcription from three promoter sequences (P1-P3). The promoter strength, conditional association of AgrA with these promoter elements and temporal delay in AgrA-mediated changes in gene expression contribute to the diversity of the quorum sensing response in different S. aureus strains. AgrA promoters comprise of imperfect direct repeats of DNA with a consensus sequence- [TA][AC][CA]GTTN[AG][TG]. Here we describe crystal structures of the DNA-binding (LytTR) domain of AgrA with different cognate DNA sequences that reveal a hitherto unanticipated feature of AgrA-DNA interactions. AgrA promoter interactions are asymmetric with fewer interactions at the binding site proximal to the -35 promoter element. Biochemical assays to evaluate AgrA-promoter interactions suggests that phosphorylation induced dimerization of AgrA can compensate for the asymmetry in AgrA-DNA interactions. The structures also provide a basis to rationalize mutations that were noted to alter AgrA activity without affecting protein-DNA interactions. Put together, the structural data, gene expression and mutational analysis reveal that promoter strength and AgrA phosphorylation enable quorum-sensing triggered transcriptional changes leading to a transition from the persistent to virulent phenotype.

Conformational features of the Staphylococcus aureus AgrA-promoter interactions rationalize quorum-sensing triggered gene expression.,Rajasree K, Fasim A, Gopal B Biochem Biophys Rep. 2016 Mar 23;6:124-134. doi: 10.1016/j.bbrep.2016.03.012., eCollection 2016 Jul. PMID:28955870[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

Loading citation details..
Citations
reviews cite this structure
No citations found

References

  1. Rajasree K, Fasim A, Gopal B. Conformational features of the Staphylococcus aureus AgrA-promoter interactions rationalize quorum-sensing triggered gene expression. Biochem Biophys Rep. 2016 Mar 23;6:124-134. doi: 10.1016/j.bbrep.2016.03.012., eCollection 2016 Jul. PMID:28955870 doi:http://dx.doi.org/10.1016/j.bbrep.2016.03.012

Contents


PDB ID 4xqj

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Personal tools