We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.

5a2h

From Proteopedia

Jump to: navigation, search

Crystal Structure of Arabidopsis thaliana Calmodulin-7

Structural highlights

5a2h is a 1 chain structure with sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.27Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CALM7_ARATH Calmodulin mediates the control of a large number of enzymes, ion channels and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Activates MPK8 in vitro.^[1]

Publication Abstract from PubMed

Calmodulin (CaM) is a Ca(2+) sensor that participates in several cellular signaling cascades by interacting with various targets, including DNA. It has been shown that Arabidopsis thaliana CaM7 (AtCaM7) interacts with Z-box DNA and functions as a transcription factor [Kushwaha R et al. (2008) Plant Cell 20, 1747-1759; Abbas N et al. (2014) Plant Cell 26, 1036-1052]. The crystal structure of AtCaM7, and a model of the AtCAM7-Z-box complex suggest that Arg-127 determines the DNA-binding ability by forming crucial interactions with the guanine base. We validated the model using biolayer interferometry, which confirmed that AtCaM7 interacts with Z-box DNA with high affinity. In contrast, the AtCaM2/3/5 isoform does not show any binding, although it differs from AtCaM7 by only a single residue.

Crystal structure of Arabidopsis thaliana calmodulin7 and insight into its mode of DNA binding.,Kumar S, Mazumder M, Gupta N, Chattopadhyay S, Gourinath S FEBS Lett. 2016 Sep;590(17):3029-39. doi: 10.1002/1873-3468.12349. Epub 2016 Aug , 24. PMID:27500568^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Takahashi F, Mizoguchi T, Yoshida R, Ichimura K, Shinozaki K. Calmodulin-dependent activation of MAP kinase for ROS homeostasis in Arabidopsis. Mol Cell. 2011 Mar 18;41(6):649-60. doi: 10.1016/j.molcel.2011.02.029. PMID:21419340 doi:http://dx.doi.org/10.1016/j.molcel.2011.02.029
↑ Kumar S, Mazumder M, Gupta N, Chattopadhyay S, Gourinath S. Crystal structure of Arabidopsis thaliana calmodulin7 and insight into its mode of DNA binding. FEBS Lett. 2016 Sep;590(17):3029-39. doi: 10.1002/1873-3468.12349. Epub 2016 Aug , 24. PMID:27500568 doi:http://dx.doi.org/10.1002/1873-3468.12349