5fva

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Toscana Virus Nucleocapsid Protein

Structural highlights

5fva is a 6 chain structure with sequence from Toscana virus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3.7Å
Ligands:NA
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NCAP_TOSV

Publication Abstract from PubMed

Toscana virus (TOSV) is an arthropod-borne virus belonging to the Phlebovirus genus within the Bunyaviridae family. As in other bunyaviruses, the genome of TOSV is made up of three RNA segments. They are encapsidated by the nucleoprotein (N), which also plays an essential role in virus replication. To date, crystallographic structures of phlebovirus N have systematically revealed closed-ring organizations which do not fully match the filamentous organization of the ribonucleoprotein (RNP) complex observed by electron microscopy. In order to further bridge the gap between crystallographic data on N and observations of the RNP by electron microscopy, the structural organization of recombinant TOSV N was investigated by an integrative approach combining X-ray diffraction crystallography, transmission electron microscopy, small-angle X-ray scattering, size-exclusion chromatography and multi-angle laser light scattering. It was found that in solution TOSV N forms open oligomers consistent with the encapsidation mechanism of phlebovirus RNA.

Toscana virus nucleoprotein oligomer organization observed in solution.,Baklouti A, Goulet A, Lichiere J, Canard B, Charrel RN, Ferron F, Coutard B, Papageorgiou N Acta Crystallogr D Struct Biol. 2017 Aug 1;73(Pt 8):650-659. doi:, 10.1107/S2059798317008774. Epub 2017 Jul 28. PMID:28777080[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
3 reviews cite this structure
Brothers in Arms: Structure, Assembly and Function of Arenaviridae Nucleoprotein.
Papageorgiou et al. (2020)
2 more
2 other articles
No citations found

See Also

References

  1. ↑ Baklouti A, Goulet A, Lichiere J, Canard B, Charrel RN, Ferron F, Coutard B, Papageorgiou N. Toscana virus nucleoprotein oligomer organization observed in solution. Acta Crystallogr D Struct Biol. 2017 Aug 1;73(Pt 8):650-659. doi:, 10.1107/S2059798317008774. Epub 2017 Jul 28. PMID:28777080 doi:http://dx.doi.org/10.1107/S2059798317008774

Contents


PDB ID 5fva

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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