5j39

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Crystal Structure of the extended TUDOR domain from TDRD2

Structural highlights

5j39 is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.95Å
Ligands:CAC, UNX
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TDRKH_HUMAN

Publication Abstract from PubMed

The P-element-induced wimpy testis (PIWI)-interacting RNA (piRNA) pathway plays a central role in transposon silencing and genome protection in the animal germline. A family of Tudor domain proteins regulates the piRNA pathway through direct Tudor domain-PIWI interactions. Tudor domains are known to fulfill this function by binding to methylated PIWI proteins in an arginine methylation-dependent manner. Here, we report a mechanism of methylation-independent Tudor domain-PIWI interaction. Unlike most other Tudor domains, the extended Tudor domain of mammalian Tudor domain-containing protein 2 (TDRD2) preferentially recognizes an unmethylated arginine-rich sequence from PIWI-like protein 1 (PIWIL1). Structural studies reveal an unexpected Tudor domain-binding mode for the PIWIL1 sequence in which the interface of Tudor and staphylococcal nuclease domains is primarily responsible for PIWIL1 peptide recognition. Mutations disrupting the TDRD2-PIWIL1 interaction compromise piRNA maturation via 3'-end trimming in vitro. Our work presented here reveals the molecular divergence of the interactions between different Tudor domain proteins and PIWI proteins.

Structural basis for arginine methylation-independent recognition of PIWIL1 by TDRD2.,Zhang H, Liu K, Izumi N, Huang H, Ding D, Ni Z, Sidhu SS, Chen C, Tomari Y, Min J Proc Natl Acad Sci U S A. 2017 Nov 8. pii: 201711486. doi:, 10.1073/pnas.1711486114. PMID:29118143[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

  1. Zhang H, Liu K, Izumi N, Huang H, Ding D, Ni Z, Sidhu SS, Chen C, Tomari Y, Min J. Structural basis for arginine methylation-independent recognition of PIWIL1 by TDRD2. Proc Natl Acad Sci U S A. 2017 Nov 8. pii: 201711486. doi:, 10.1073/pnas.1711486114. PMID:29118143 doi:http://dx.doi.org/10.1073/pnas.1711486114

Contents


PDB ID 5j39

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