5k5r

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AspA-32mer DNA,crystal form 2

Structural highlights

5k5r is a 8 chain structure with sequence from Sulfolobus sp. NOB8H2 and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3.09Å
Ligands:PO4
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

O93706_9CREN

Publication Abstract from PubMed

Although recent studies have provided a wealth of information about archaeal biology, nothing is known about the molecular basis of DNA segregation in these organisms. Here, we unveil the machinery and assembly mechanism of the archaeal Sulfolobus pNOB8 partition system. This system uses three proteins: ParA; an atypical ParB adaptor; and a centromere-binding component, AspA. AspA utilizes a spreading mechanism to create a DNA superhelix onto which ParB assembles. This supercomplex links to the ParA motor, which contains a bacteria-like Walker motif. The C domain of ParB harbors structural similarity to CenpA, which dictates eukaryotic segregation. Thus, this archaeal system combines bacteria-like and eukarya-like components, which suggests the possible conservation of DNA segregation principles across the three domains of life.

Structures of archaeal DNA segregation machinery reveal bacterial and eukaryotic linkages.,Schumacher MA, Tonthat NK, Lee J, Rodriguez-Castaneda FA, Chinnam NB, Kalliomaa-Sanford AK, Ng IW, Barge MT, Shaw PL, Barilla D Science. 2015 Sep 4;349(6252):1120-4. doi: 10.1126/science.aaa9046. PMID:26339031[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
7 reviews cite this structure
Wagner et al. (2017)
No citations found

References

  1. Schumacher MA, Tonthat NK, Lee J, Rodriguez-Castaneda FA, Chinnam NB, Kalliomaa-Sanford AK, Ng IW, Barge MT, Shaw PL, Barilla D. Structures of archaeal DNA segregation machinery reveal bacterial and eukaryotic linkages. Science. 2015 Sep 4;349(6252):1120-4. doi: 10.1126/science.aaa9046. PMID:26339031 doi:http://dx.doi.org/10.1126/science.aaa9046

Contents


PDB ID 5k5r

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