5kny

Structural highlights

Function

HGPRT_MYCTU Purine salvage pathway enzyme that catalyzes the transfer of the ribosyl-5-phosphate group from 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to the N9 position of the 6-oxopurines hypoxanthine and guanine to form the corresponding ribonucleotides IMP (inosine 5'-monophosphate) and GMP (guanosine 5'-monophosphate), with the release of PPi (PubMed:19362594, PubMed:25915781). Thus, specifically recycles hypoxanthine and guanine imported from the external medium, and converts them to IMP and GMP, respectively. Cannot use xanthine as substrate (PubMed:19362594).^{[1] [2]}

See Also

• Phosphoribosyltransferase 3D structures

References

1. ↑ Biazus G, Schneider CZ, Palma MS, Basso LA, Santos DS. Hypoxanthine-guanine phosphoribosyltransferase from Mycobacterium tuberculosis H37Rv: cloning, expression, and biochemical characterization. Protein Expr Purif. 2009 Aug;66(2):185-90. PMID:19362594 doi:10.1016/j.pep.2009.04.001
2. ↑ Eng WS, Hockova D, Spacek P, Janeba Z, West NP, Woods K, Naesens LM, Keough DT, Guddat LW. First Crystal Structures of Mycobacterium tuberculosis 6-Oxopurine Phosphoribosyltransferase: Complexes with GMP and Pyrophosphate and with Acyclic Nucleoside Phosphonates Whose Prodrugs Have Antituberculosis Activity. J Med Chem. 2015 May 13. PMID:25915781 doi:http://dx.doi.org/10.1021/acs.jmedchem.5b00611