5l4e

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X-ray structure of the 2-22' locally-closed mutant of GLIC in complex with thiopental

Structural highlights

5l4e is a 5 chain structure with sequence from Gloeobacter. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3.5Å
Ligands:CL, D12, EDP
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GLIC_GLOVI Cationic channel with similar permeabilities for Na(+) and K(+), that is activated by an increase of the proton concentration on the extracellular side. Displays no permeability for chloride ions. Shows slow kinetics of activation, no desensitization and a single channel conductance of 8 pS. Might contribute to adaptation to external pH change.[1]

Publication Abstract from PubMed

Barbiturates induce anesthesia by modulating the activity of anionic and cationic pentameric ligand-gated ion channels (pLGICs). Despite more than a century of use in clinical practice, the prototypic binding site for this class of drugs within pLGICs is yet to be described. In this study, we present the first X-ray structures of barbiturates bound to GLIC, a cationic prokaryotic pLGIC with excellent structural homology to other relevant channels sensitive to general anesthetics and, as shown here, to barbiturates, at clinically-relevant concentrations. Several derivatives of barbiturates containing anomalous scatterers were synthesized and helped to unambiguously identify a unique barbiturate binding site within the central ion channel pore in a closed conformation. In addition, docking calculations around the observed binding site for all three states of the receptor, including a model of the desensitized state, showed that barbiturates preferentially stabilize the closed state. The identification of this pore binding site sheds light on the mechanism of barbiturates inhibition of cationic pLGICs and allows the rationalisation of several structural and functional features previously observed for barbiturates.

Barbiturates bind in the GLIC ion channel pore and cause inhibition by stabilizing a closed state.,Fourati Z, Ruza RR, Laverty D, Drege E, Delarue-Cochin S, Joseph D, Koehl P, Smart T, Delarue M J Biol Chem. 2016 Dec 16. pii: jbc.M116.766964. PMID:27986812[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
2 reviews cite this structure
Towards a Comprehensive Understanding of Anesthetic Mechanisms of Action: A Decade of Discovery.
Hemmings et al. (2019)
1 more
9 other articles
No citations found

See Also

References

  1. ↑ Bocquet N, Prado de Carvalho L, Cartaud J, Neyton J, Le Poupon C, Taly A, Grutter T, Changeux JP, Corringer PJ. A prokaryotic proton-gated ion channel from the nicotinic acetylcholine receptor family. Nature. 2007 Jan 4;445(7123):116-9. Epub 2006 Dec 10. PMID:17167423 doi:10.1038/nature05371
  2. ↑ Fourati Z, Ruza RR, Laverty D, Drege E, Delarue-Cochin S, Joseph D, Koehl P, Smart T, Delarue M. Barbiturates bind in the GLIC ion channel pore and cause inhibition by stabilizing a closed state. J Biol Chem. 2016 Dec 16. pii: jbc.M116.766964. PMID:27986812 doi:http://dx.doi.org/10.1074/jbc.M116.766964

Contents


PDB ID 5l4e

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