Structural highlights
5l6v is a 16 chain structure with sequence from Escherichia coli K-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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Method: | X-ray diffraction, Resolution 2.667Å |
Ligands: | , , , , |
Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
GLGC_ECOLI Catalyzes the synthesis of ADP-glucose, a sugar donor used in elongation reactions on alpha-glucans.
Publication Abstract from PubMed
ADP-glucose pyrophosphorylase (AGPase) catalyzes the rate-limiting step of bacterial glycogen and plant starch biosynthesis, the most common carbon storage polysaccharides in nature. A major challenge is to understand how AGPase activity is regulated by metabolites in the energetic flux within the cell. Here we report crystal structures of the homotetrameric AGPase from Escherichia coli in complex with its physiological positive and negative allosteric regulators, fructose-1,6-bisphosphate (FBP) and AMP, and sucrose in the active site. FBP and AMP bind to partially overlapping sites located in a deep cleft between glycosyltransferase A-like and left-handed beta helix domains of neighboring protomers, accounting for the fact that sensitivity to inhibition by AMP is modulated by the concentration of the activator FBP. We propose a model in which the energy reporters regulate EcAGPase catalytic activity by intra-protomer interactions and inter-protomer crosstalk, with a sensory motif and two regulatory loops playing a prominent role.
Structural Basis of Glycogen Biosynthesis Regulation in Bacteria.,Cifuente JO, Comino N, Madariaga-Marcos J, Lopez-Fernandez S, Garcia-Alija M, Agirre J, Albesa-Jove D, Guerin ME Structure. 2016 Sep 6;24(9):1613-1622. doi: 10.1016/j.str.2016.06.023. Epub 2016 , Aug 18. PMID:27545622[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Cifuente JO, Comino N, Madariaga-Marcos J, Lopez-Fernandez S, Garcia-Alija M, Agirre J, Albesa-Jove D, Guerin ME. Structural Basis of Glycogen Biosynthesis Regulation in Bacteria. Structure. 2016 Sep 6;24(9):1613-1622. doi: 10.1016/j.str.2016.06.023. Epub 2016 , Aug 18. PMID:27545622 doi:http://dx.doi.org/10.1016/j.str.2016.06.023