5luf

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Cryo-EM of bovine respirasome

Structural highlights

5luf is a 20 chain structure with sequence from Bos taurus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Electron Microscopy, Resolution 9.1Å
Experimental data:Check to display Experimental Data
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

QCR1_BOVIN This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This protein may mediate formation of the complex between cytochromes c and c1.

Publication Abstract from PubMed

Respirasomes are macromolecular assemblies of the respiratory chain complexes I, III and IV in the inner mitochondrial membrane. We determined the structure of supercomplex I1III2IV1 from bovine heart mitochondria by cryo-EM at 9 A resolution. Most protein-protein contacts between complex I, III and IV in the membrane are mediated by supernumerary subunits. Of the two Rieske iron-sulfur cluster domains in the complex III dimer, one is resolved, indicating that this domain is immobile and unable to transfer electrons. The central position of the active complex III monomer between complex I and IV in the respirasome is optimal for accepting reduced quinone from complex I over a short diffusion distance of 11 nm, and delivering reduced cytochrome c to complex IV. The functional asymmetry of complex III provides strong evidence for directed electron flow from complex I to complex IV through the active complex III monomer in the mammalian supercomplex.

Functional asymmetry and electron flow in the bovine respirasome.,Sousa JS, Mills DJ, Vonck J, Kuhlbrandt W Elife. 2016 Nov 10;5. pii: e21290. doi: 10.7554/eLife.21290. PMID:27830641[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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See Also

References

  1. Sousa JS, Mills DJ, Vonck J, Kuhlbrandt W. Functional asymmetry and electron flow in the bovine respirasome. Elife. 2016 Nov 10;5. pii: e21290. doi: 10.7554/eLife.21290. PMID:27830641 doi:http://dx.doi.org/10.7554/eLife.21290

Contents


5luf, resolution 9.10Å

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