5m94

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Crystal structure of Staphylococcus capitis divalent metal ion transporter (DMT) in complex with nanobody

Structural highlights

5m94 is a 4 chain structure with sequence from Lama glama and Staphylococcus capitis. This structure supersedes the now removed PDB entry 4wgv. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

Members of the SLC11 (NRAMP) family transport iron and other transition-metal ions across cellular membranes. These membrane proteins are present in all kingdoms of life with a high degree of sequence conservation. To gain insight into the determinants of ion selectivity, we have determined the crystal structure of Staphylococcus capitis DMT (ScaDMT), a close prokaryotic homolog of the family. ScaDMT shows a familiar architecture that was previously identified in the amino acid permease LeuT. The protein adopts an inward-facing conformation with a substrate-binding site located in the center of the transporter. This site is composed of conserved residues, which coordinate Mn2+, Fe2+ and Cd2+ but not Ca2+. Mutations of interacting residues affect ion binding and transport in both ScaDMT and human DMT1. Our study thus reveals a conserved mechanism for transition-metal ion selectivity within the SLC11 family.

Crystal structure of a SLC11 (NRAMP) transporter reveals the basis for transition-metal ion transport.,Ehrnstorfer IA, Geertsma ER, Pardon E, Steyaert J, Dutzler R Nat Struct Mol Biol. 2014 Oct 19. doi: 10.1038/nsmb.2904. PMID:25326704[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
20 reviews cite this structure
Targeting iron metabolism in drug discovery and delivery.
Crielaard et al. (2017)
19 more
72 other articles
No citations found

See Also

References

  1. ↑ Ehrnstorfer IA, Geertsma ER, Pardon E, Steyaert J, Dutzler R. Crystal structure of a SLC11 (NRAMP) transporter reveals the basis for transition-metal ion transport. Nat Struct Mol Biol. 2014 Oct 19. doi: 10.1038/nsmb.2904. PMID:25326704 doi:http://dx.doi.org/10.1038/nsmb.2904

Contents


PDB ID 5m94

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