5mdv

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Structure of ArfA and RF2 bound to the 70S ribosome (accommodated state)

Structural highlights

5mdv is a 10 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Electron Microscopy, Resolution 2.97Å
Ligands:MG, FME, ZN
Experimental data:Check to display Experimental Data
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ARFA_ECOLI Rescues the ribosomes stalled at the 3' end of non-stop mRNAs. May induce hydrolysis of the ribosome-bound peptidyl-tRNA. This activity is crucial when the stalled ribosome cannot be rescued by the SsrA(tmRNA)-SmpB quality control system.[1] [2]

Publication Abstract from PubMed

Ribosomes stall when they encounter the end of messenger RNA (mRNA) without an in-frame stop codon. In bacteria, these "nonstop" complexes can be rescued by alternative ribosome-rescue factor A (ArfA). We used electron cryomicroscopy to determine structures of ArfA bound to the ribosome with 3'-truncated mRNA, at resolutions ranging from 3.0 to 3.4 angstroms. ArfA binds within the ribosomal mRNA channel and substitutes for the absent stop codon in the A site by specifically recruiting release factor 2 (RF2), initially in a compact preaccommodated state. A similar conformation of RF2 may occur on stop codons, suggesting a general mechanism for release-factor-mediated translational termination in which a conformational switch leads to peptide release only when the appropriate signal is present in the A site.

Translational termination without a stop codon.,James NR, Brown A, Gordiyenko Y, Ramakrishnan V Science. 2016 Dec 16;354(6318):1437-1440. Epub 2016 Dec 1. PMID:27934701[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
8 reviews cite this structure
Quality controls induced by aberrant translation.
Inada et al. (2020)
7 more
28 other articles
No citations found

See Also

References

  1. ↑ Chadani Y, Ono K, Ozawa S, Takahashi Y, Takai K, Nanamiya H, Tozawa Y, Kutsukake K, Abo T. Ribosome rescue by Escherichia coli ArfA (YhdL) in the absence of trans-translation system. Mol Microbiol. 2010 Nov;78(4):796-808. doi: 10.1111/j.1365-2958.2010.07375.x., Epub 2010 Sep 24. PMID:21062370 doi:http://dx.doi.org/10.1111/j.1365-2958.2010.07375.x
  2. ↑ Garza-Sanchez F, Schaub RE, Janssen BD, Hayes CS. tmRNA regulates synthesis of the ArfA ribosome rescue factor. Mol Microbiol. 2011 Jun;80(5):1204-19. doi: 10.1111/j.1365-2958.2011.07638.x., Epub 2011 Mar 30. PMID:21435036 doi:http://dx.doi.org/10.1111/j.1365-2958.2011.07638.x
  3. ↑ James NR, Brown A, Gordiyenko Y, Ramakrishnan V. Translational termination without a stop codon. Science. 2016 Dec 16;354(6318):1437-1440. Epub 2016 Dec 1. PMID:27934701 doi:http://dx.doi.org/10.1126/science.aai9127

Contents


Creating or updating UnitsVisual [2/57]

5mdv, resolution 2.97Å

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