5mhj

Structural highlights

Function

ICP4_HHV11 Plays an essential role in the regulation of viral gene expression by both activating and repressing host RNA polymerase II-mediated transcription. Binds with high affinity to the sequence 5'-ATCGTC-3'. Activates transcription by recruiting a form of the host TFIID to promoters and stabilizing the pre-initiation complex formation. Negatively regulates its own transcription. This immediate early (IE) protein is absolutely necessary for the transition from IE transcription to later viral gene transcription.^{[1] [2] [3] [4] [5] [6] [7]}

References

1. ↑ Sampath P, Deluca NA. Binding of ICP4, TATA-binding protein, and RNA polymerase II to herpes simplex virus type 1 immediate-early, early, and late promoters in virus-infected cells. J Virol. 2008 Mar;82(5):2339-49. Epub 2007 Dec 19. PMID:18094162 doi:http://dx.doi.org/10.1128/JVI.02459-07
2. ↑ Zabierowski SE, Deluca NA. Stabilized binding of TBP to the TATA box of herpes simplex virus type 1 early (tk) and late (gC) promoters by TFIIA and ICP4. J Virol. 2008 Apr;82(7):3546-54. doi: 10.1128/JVI.02560-07. Epub 2008 Jan 23. PMID:18216093 doi:http://dx.doi.org/10.1128/JVI.02560-07
3. ↑ Papavassiliou AG, Wilcox KW, Silverstein SJ. The interaction of ICP4 with cell/infected-cell factors and its state of phosphorylation modulate differential recognition of leader sequences in herpes simplex virus DNA. EMBO J. 1991 Feb;10(2):397-406. PMID:1846804
4. ↑ Wagner LM, Lester JT, Sivrich FL, DeLuca NA. The N terminus and C terminus of herpes simplex virus 1 ICP4 cooperate to activate viral gene expression. J Virol. 2012 Jun;86(12):6862-74. doi: 10.1128/JVI.00651-12. Epub 2012 Apr 11. PMID:22496239 doi:http://dx.doi.org/10.1128/JVI.00651-12
5. ↑ Wagner LM, Bayer A, Deluca NA. Requirement of the N-terminal activation domain of herpes simplex virus ICP4 for viral gene expression. J Virol. 2013 Jan;87(2):1010-8. doi: 10.1128/JVI.02844-12. Epub 2012 Nov 7. PMID:23135715 doi:http://dx.doi.org/10.1128/JVI.02844-12
6. ↑ Wagner LM, DeLuca NA. Temporal association of herpes simplex virus ICP4 with cellular complexes functioning at multiple steps in PolII transcription. PLoS One. 2013 Oct 11;8(10):e78242. doi: 10.1371/journal.pone.0078242., eCollection 2013. PMID:24147125 doi:http://dx.doi.org/10.1371/journal.pone.0078242
7. ↑ Carrozza MJ, DeLuca NA. Interaction of the viral activator protein ICP4 with TFIID through TAF250. Mol Cell Biol. 1996 Jun;16(6):3085-93. PMID:8649420