5my1

Publication Abstract from PubMed

DNA transcription is functionally coupled to messenger RNA (mRNA) translation in bacteria, but how this is achieved remains unclear. Here we show that RNA polymerase (RNAP) and the ribosome of Escherichia coli can form a defined transcribing and translating "expressome" complex. The cryo-electron microscopic structure of the expressome reveals continuous protection of ~30 nucleotides of mRNA extending from the RNAP active center to the ribosome decoding center. The RNAP-ribosome interface includes the RNAP subunit alpha carboxyl-terminal domain, which is required for RNAP-ribosome interaction in vitro and for pronounced cell growth defects upon translation inhibition in vivo, consistent with its function in transcription-translation coupling. The expressome structure can only form during transcription elongation and explains how translation can prevent transcriptional pausing, backtracking, and termination.

Architecture of a transcribing-translating expressome.,Kohler R, Mooney RA, Mills DJ, Landick R, Cramer P Science. 2017 Apr 14;356(6334):194-197. doi: 10.1126/science.aal3059. PMID:28408604^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.