5u6n

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Crystal structure of UDP-glucosyltransferase, UGT74F2 (T15S), with UDP and salicylic acid

Structural highlights

5u6n is a 2 chain structure with sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.001Å
Ligands:BGC, GLN, PRD_900024, SAL, UDP
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

U74F2_ARATH Glycosyltransferase that glucosylates benzoic acid and derivatives. Substrate preference is benzoic acid > salicylic acid (SA) > 3-hydroxybenzoic acid > 4-hydroxybenzoic acid. Catalyzes the formation of both SA 2-O-beta-D-glucoside (SAG) and SA glucose ester (SGE). Has high affinity for the tryptophan precursor anthranilate. Catalyzes the formation of anthranilate glucose ester. Is the major source of this activity in the plant.[1] [2] [3] [4] [5]

See Also

References

  1. ↑ Lim EK, Doucet CJ, Li Y, Elias L, Worrall D, Spencer SP, Ross J, Bowles DJ. The activity of Arabidopsis glycosyltransferases toward salicylic acid, 4-hydroxybenzoic acid, and other benzoates. J Biol Chem. 2002 Jan 4;277(1):586-92. Epub 2001 Oct 18. PMID:11641410 doi:http://dx.doi.org/10.1074/jbc.M109287200
  2. ↑ Quiel JA, Bender J. Glucose conjugation of anthranilate by the Arabidopsis UGT74F2 glucosyltransferase is required for tryptophan mutant blue fluorescence. J Biol Chem. 2003 Feb 21;278(8):6275-81. Epub 2002 Dec 9. PMID:12475971 doi:http://dx.doi.org/10.1074/jbc.M211822200
  3. ↑ Song JT. Induction of a salicylic acid glucosyltransferase, AtSGT1, is an early disease response in Arabidopsis thaliana. Mol Cells. 2006 Oct 31;22(2):233-8. PMID:17085977
  4. ↑ Song JT, Koo YJ, Seo HS, Kim MC, Choi YD, Kim JH. Overexpression of AtSGT1, an Arabidopsis salicylic acid glucosyltransferase, leads to increased susceptibility to Pseudomonas syringae. Phytochemistry. 2008 Mar;69(5):1128-34. doi: 10.1016/j.phytochem.2007.12.010., Epub 2008 Jan 28. PMID:18226820 doi:http://dx.doi.org/10.1016/j.phytochem.2007.12.010
  5. ↑ Eudes A, Bozzo GG, Waller JC, Naponelli V, Lim EK, Bowles DJ, Gregory JF 3rd, Hanson AD. Metabolism of the folate precursor p-aminobenzoate in plants: glucose ester formation and vacuolar storage. J Biol Chem. 2008 May 30;283(22):15451-9. doi: 10.1074/jbc.M709591200. Epub 2008 , Apr 2. PMID:18385129 doi:http://dx.doi.org/10.1074/jbc.M709591200

Contents


PDB ID 5u6n

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OCA

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