5ybh

Structural highlights

Function

SCTN_SHIFL ATPase component of the type III secretion system (T3SS), also called injectisome, which is used to inject bacterial effector proteins into eukaryotic host cells (PubMed:26947936, PubMed:27770024, PubMed:29595954). Acts as a molecular motor to provide the energy that is required for the export of proteins (Probable). Required for type III secretion apparatus (T3SA) formation, proper protein secretion, host cell invasion and virulence (PubMed:26947936, PubMed:27770024, PubMed:31162724). May play a critical role in T3SS substrate recognition, disassembly of the effector/chaperone complex and unfolding of the effector in an ATP-dependent manner prior to secretion (By similarity).[UniProtKB:P0A1B9]^{[1] [2] [3] [4] [5] [6]}

References

1. ↑ Burgess JL, Jones HB, Kumar P, Toth RT 4th, Middaugh CR, Antony E, Dickenson NE. Spa47 is an oligomerization-activated type three secretion system (T3SS) ATPase from Shigella flexneri. Protein Sci. 2016 May;25(5):1037-48. PMID:26947936 doi:10.1002/pro.2917
2. ↑ Burgess JL, Burgess RA, Morales Y, Bouvang JM, Johnson SJ, Dickenson NE. Structural and Biochemical Characterization of Spa47 Provides Mechanistic Insight into Type III Secretion System ATPase Activation and Shigella Virulence Regulation. J Biol Chem. 2016 Oct 21. pii: jbc.M116.755256. PMID:27770024 doi:http://dx.doi.org/10.1074/jbc.M116.755256
3. ↑ Case HB, Dickenson NE. MxiN Differentially Regulates Monomeric and Oligomeric Species of the Shigella Type Three Secretion System ATPase Spa47. Biochemistry. 2018 Apr 17;57(15):2266-2277. PMID:29595954 doi:10.1021/acs.biochem.8b00070
4. ↑ Demler HJ, Case HB, Morales Y, Bernard AR, Johnson SJ, Dickenson NE. Interfacial amino acids support Spa47 oligomerization and shigella type three secretion system activation. Proteins. 2019 Jun 4. doi: 10.1002/prot.25754. PMID:31162724 doi:http://dx.doi.org/10.1002/prot.25754
5. ↑ Burgess JL, Jones HB, Kumar P, Toth RT 4th, Middaugh CR, Antony E, Dickenson NE. Spa47 is an oligomerization-activated type three secretion system (T3SS) ATPase from Shigella flexneri. Protein Sci. 2016 May;25(5):1037-48. PMID:26947936 doi:10.1002/pro.2917
6. ↑ Burgess JL, Burgess RA, Morales Y, Bouvang JM, Johnson SJ, Dickenson NE. Structural and Biochemical Characterization of Spa47 Provides Mechanistic Insight into Type III Secretion System ATPase Activation and Shigella Virulence Regulation. J Biol Chem. 2016 Oct 21. pii: jbc.M116.755256. PMID:27770024 doi:http://dx.doi.org/10.1074/jbc.M116.755256