5yi7

From Proteopedia

Crystal structure of drosophila Numb PTB domain and Pon peptide complex

PDB ID 5yi7

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Structural highlights

5yi7 is a 4 chain structure with sequence from Drosophila melanogaster. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.7Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NUMB_DROME Required in determination of cell fate during sensory organ formation in embryos. Functions in nuclei and seems to interact with nucleic acids.^[1]

Publication Abstract from PubMed

Uneven distribution and local concentration of protein complexes on distinct membrane cortices is a fundamental property in numerous biological processes, including Drosophila neuroblast (NB) asymmetric cell divisions and cell polarity in general. In NBs, the cell fate determinant Numb forms a basal crescent together with Pon and is segregated into the basal daughter cell to initiate its differentiation. Here we discover that Numb PTB domain, using two distinct binding surfaces, recognizes repeating motifs within Pon in a previously unrecognized mode. The multivalent Numb-Pon interaction leads to high binding specificity and liquid-liquid phase separation of the complex. Perturbations of the Numb/Pon complex phase transition impair the basal localization of Numb and its subsequent suppression of Notch signaling during NB asymmetric divisions. Such phase-transition-mediated protein condensations on distinct membrane cortices may be a general mechanism for various cell polarity regulatory complexes.

Basal condensation of Numb and Pon complex via phase transition during Drosophila neuroblast asymmetric division.,Shan Z, Tu Y, Yang Y, Liu Z, Zeng M, Xu H, Long J, Zhang M, Cai Y, Wen W Nat Commun. 2018 Feb 21;9(1):737. doi: 10.1038/s41467-018-03077-3. PMID:29467404^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Uemura T, Shepherd S, Ackerman L, Jan LY, Jan YN. numb, a gene required in determination of cell fate during sensory organ formation in Drosophila embryos. Cell. 1989 Jul 28;58(2):349-60. PMID:2752427
↑ Shan Z, Tu Y, Yang Y, Liu Z, Zeng M, Xu H, Long J, Zhang M, Cai Y, Wen W. Basal condensation of Numb and Pon complex via phase transition during Drosophila neuroblast asymmetric division. Nat Commun. 2018 Feb 21;9(1):737. doi: 10.1038/s41467-018-03077-3. PMID:29467404 doi:http://dx.doi.org/10.1038/s41467-018-03077-3