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5yzz

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AtVAL1 B3 domain in complex with 13bp-DNA

Structural highlights

5yzz is a 3 chain structure with sequence from Arabidopsis thaliana and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.58Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

VAL1_ARATH Transcriptional repressor of gene expression involved in embryonic pathways, such as LEC1, ABI3, and FUS3. Repressor of the sugar-inducible genes involved in the seed maturation program in seedlings. Plays an essential role in regulating the transition from seed maturation to seedling growth. Functionally redundant with VAL2/HSL1.^[1] ^[2] ^[3]

Publication Abstract from PubMed

Vernalization is a pivotal stage for some plants involving many epigenetic changes during cold exposure. In Arabidopsis, an essential step in vernalization for further flowering is successful silence the potent floral repressor Flowering Locus C (FLC) by repressing histone mark. AtVal1 is a multi-function protein containing five domains that participate into many recognition processes and is validated to recruit the repress histone modifier PHD-PRC2 complex and interact with components of the ASAP complex target to the FLC nucleation region through recognizing a cis element known as CME (cold memory element) by its plant-specific B3 domain. Here, we determine the crystal structure of the B3 domain in complex with Sph/RY motif in CME. Our structural analysis reveals the specific DNA recognition by B3 domain, combined with our in vitro experiments, we provide the structural insight into the important implication of AtVAL1-B3 domain in flowering process.

Structural insight into the role of VAL1 B3 domain for targeting to FLC locus in Arabidopsis thaliana.,Wu B, Zhang M, Su S, Liu H, Gan J, Ma J Biochem Biophys Res Commun. 2018 Jun 22;501(2):415-422. doi:, 10.1016/j.bbrc.2018.05.002. Epub 2018 May 10. PMID:29733847^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Tsukagoshi H, Saijo T, Shibata D, Morikami A, Nakamura K. Analysis of a sugar response mutant of Arabidopsis identified a novel B3 domain protein that functions as an active transcriptional repressor. Plant Physiol. 2005 Jun;138(2):675-85. Epub 2005 May 13. PMID:15894743 doi:http://dx.doi.org/pp.104.057752
↑ Suzuki M, Wang HH, McCarty DR. Repression of the LEAFY COTYLEDON 1/B3 regulatory network in plant embryo development by VP1/ABSCISIC ACID INSENSITIVE 3-LIKE B3 genes. Plant Physiol. 2007 Feb;143(2):902-11. doi: 10.1104/pp.106.092320. Epub 2006 Dec , 8. PMID:17158584 doi:http://dx.doi.org/10.1104/pp.106.092320
↑ Tsukagoshi H, Morikami A, Nakamura K. Two B3 domain transcriptional repressors prevent sugar-inducible expression of seed maturation genes in Arabidopsis seedlings. Proc Natl Acad Sci U S A. 2007 Feb 13;104(7):2543-7. doi:, 10.1073/pnas.0607940104. Epub 2007 Jan 31. PMID:17267611 doi:http://dx.doi.org/10.1073/pnas.0607940104
↑ Wu B, Zhang M, Su S, Liu H, Gan J, Ma J. Structural insight into the role of VAL1 B3 domain for targeting to FLC locus in Arabidopsis thaliana. Biochem Biophys Res Commun. 2018 Jun 22;501(2):415-422. doi:, 10.1016/j.bbrc.2018.05.002. Epub 2018 May 10. PMID:29733847 doi:http://dx.doi.org/10.1016/j.bbrc.2018.05.002