6axp
From Proteopedia
Structure of cetuximab with aminoheptanoic acid-linked n-octylarginine meditope variant
Structural highlights
DiseaseIGKC_HUMAN Defects in IGKC are the cause of immunoglobulin kappa light chain deficiency (IGKCD) [MIM:614102. IGKCD is a disease characterized by the complete absence of immunoglobulin kappa chains.[1] FunctionPublication Abstract from PubMedMeditope, a cyclic 12-residue peptide, binds to a unique binding side between the light and heavy chains of the cetuximab Fab. In an effort to improve the affinity of the interaction, it was sought to extend the side chain of Arg8 in the meditope, a residue that is accessible from the other side of the meditope binding site, in order to increase the number of interactions. These modifications included an n-butyl and n-octyl extension as well as hydroxyl, amine and carboxyl substitutions. The atomic structures of the complexes and the binding kinetics for each modified meditope indicated that each extension threaded through the Fab `hole' and that the carboxyethylarginine substitution makes a favorable interaction with the Fab, increasing the half-life of the complex by threefold compared with the unmodified meditope. Taken together, these studies provide a basis for the design of additional modifications to enhance the overall affinity of this unique interaction. Meditope-Fab interaction: threading the hole.,Bzymek KP, Ma Y, Avery KN, Horne DA, Williams JC Acta Crystallogr F Struct Biol Commun. 2017 Dec 1;73(Pt 12):688-694. doi:, 10.1107/S2053230X17016272. Epub 2017 Nov 18. PMID:29199990[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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