| Structural highlights
6f6f is a 1 chain structure with sequence from Geoka. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| Ligands: | , , , |
Related: | 4hr0, 4hr4, 4hr5, 4xb9, 4xbv, 4xbw, 5dco, 5dcr, 5dcs, 5ekb, 5omk, 5omj, 6f65, 6f6b, 6f6c, 6f6e, 6f6g, 6f6h, 6f6k, 6f6l, 6f6m |
Gene: | GK2771 (GEOKA) |
Activity: | Ribonucleoside-diphosphate reductase, with EC number 1.17.4.1 |
Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Publication Abstract from PubMed
R2-like ligand-binding oxidases contain a dinuclear metal cofactor which can consist either of two iron ions or one manganese and one iron ion, but the heterodinuclear Mn/Fe cofactor is the preferred assembly in the presence of Mn(II) and Fe(II) in vitro. We have previously shown that both types of cofactor are capable of catalyzing formation of a tyrosine-valine ether cross-link in the protein scaffold. Here we demonstrate that Mn/Fe centers catalyze cross-link formation more efficiently than Fe/Fe centers, indicating that the heterodinuclear cofactor is the biologically relevant one. We further explore the chemical potential of the Mn/Fe cofactor by introducing mutations at the cross-linking valine residue. We find that cross-link formation is possible also to the tertiary beta-carbon in an isoleucine, but not to the secondary beta-carbon or tertiary gamma-carbon in a leucine, nor to the primary beta-carbon of an alanine. These results illustrate that the reactivity of the cofactor is highly specific and directed.
Ether cross-link formation in the R2-like ligand-binding oxidase.,Griese JJ, Branca RMM, Srinivas V, Hogbom M J Biol Inorg Chem. 2018 Jun 26. pii: 10.1007/s00775-018-1583-3. doi:, 10.1007/s00775-018-1583-3. PMID:29946980[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Griese JJ, Branca RMM, Srinivas V, Hogbom M. Ether cross-link formation in the R2-like ligand-binding oxidase. J Biol Inorg Chem. 2018 Jun 26. pii: 10.1007/s00775-018-1583-3. doi:, 10.1007/s00775-018-1583-3. PMID:29946980 doi:http://dx.doi.org/10.1007/s00775-018-1583-3
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