Structural highlights
6fdb is a 8 chain structure with sequence from "bacillus_cholerae-suis"_smith_1894 "bacillus cholerae-suis" smith 1894. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Gene: | A7S24_10320, A7S72_11360, BUJ19_10850, CBI64_04620, CEP91_14370, IN36_13565, IN77_10790, IN95_23850, NGUA18_00608 ("Bacillus cholerae-suis" Smith 1894) |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Publication Abstract from PubMed
Oligonucleotide therapeutics have transformative potential in modern medicine but are poor drug candidates in themselves unless fitted with compensatory carrier systems. We describe a simple approach to transform a designed porous protein cage into a nucleic acid delivery vehicle. By introducing arginine mutations to the lumenal surface, a positively supercharged capsule is created, which can encapsidate oligonucleotides in vitro with high binding affinity. We demonstrate that the siRNA-loaded cage is taken up by mammalian cells and releases its cargo to induce RNAi and knockdown gene expression. These general concepts could also be applied to alternative scaffold designs, expediting the development of artificial protein cages toward delivery applications.
Rational Engineering of a Designed Protein Cage for siRNA Delivery.,Edwardson TGW, Mori T, Hilvert D J Am Chem Soc. 2018 Aug 9. doi: 10.1021/jacs.8b06442. PMID:30091604[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Edwardson TGW, Mori T, Hilvert D. Rational Engineering of a Designed Protein Cage for siRNA Delivery. J Am Chem Soc. 2018 Aug 9. doi: 10.1021/jacs.8b06442. PMID:30091604 doi:http://dx.doi.org/10.1021/jacs.8b06442
