6flu

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Photorhabdus asymbiotica lectin (PHL) in complex with synthetic C-fucoside

Structural highlights

6flu is a 2 chain structure with sequence from Photorhabdus asymbiotica subsp. asymbiotica ATCC 43949. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.78Å
Ligands:CL, DTK, NA
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

C7BLE4_PHOAA

Publication Abstract from PubMed

A recently described bangle lectin (PHL) from the bacterium Photorhabdus asymbiotica was identified as a mainly fucose-binding protein that could play an important role in the host-pathogen interaction and in the modulation of host immune response. Structural studies showed that PHL is a homo-dimer that contains up to seven L-fucose-specific binding sites per monomer. For these reasons, potential ligands of the PHL lectin: alpha-L-fucopyranosyl-containing mono-, di-, tetra-, hexa- and dodecavalent ligands were tested. Two types of polyvalent structures were investigated - calix[4]arenes and dendrimers. The shared feature of all these structures was a C-glycosidic bond instead of the more common but physiologically unstable O-glycosidic bond. The inhibition potential of the tested structures was assessed using different techniques - hemagglutination, surface plasmon resonance, isothermal titration calorimetry, and cell cross-linking. All the ligands proved to be better than free L-fucose. The most active hexavalent dendrimer exhibited affinity three orders of magnitude higher than that of standard L-fucose. To determine the binding mode of some ligands, crystal complex PHL/fucosides 2 - 4 were prepared and studied using X-ray crystallography. The electron density in complexes proved the presence of the compounds in 6 out of 7 fucose-binding sites.

Fucosylated inhibitors of recently identified bangle lectin from Photorhabdus asymbiotica.,Paulikova G, Houser J, Kasakova M, Oroszova B, Bertolotti B, Parkan K, Moravcova J, Wimmerova M Sci Rep. 2019 Oct 17;9(1):14904. doi: 10.1038/s41598-019-51357-9. PMID:31624296[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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References

  1. Paulikova G, Houser J, Kasakova M, Oroszova B, Bertolotti B, Parkan K, Moravcova J, Wimmerova M. Fucosylated inhibitors of recently identified bangle lectin from Photorhabdus asymbiotica. Sci Rep. 2019 Oct 17;9(1):14904. doi: 10.1038/s41598-019-51357-9. PMID:31624296 doi:http://dx.doi.org/10.1038/s41598-019-51357-9

Contents


PDB ID 6flu

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