6fsf

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Crystal structure of the tandem PX-PH-domains of Bem3 from Saccharomyces cerevisiae

Structural highlights

6fsf is a 1 chain structure with sequence from Saccharomyces cerevisiae S288C. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.2Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

BEM3_YEAST GTPase-activating protein (GAP) for CDC42 and less efficiently for RHO1. Negative regulator of the pheromone-response pathway through the STE20 protein kinase.[1] [2]

Publication Abstract from PubMed

The structure of the tandem lipid-binding PX and pleckstrin-homology (PH) domains of the Cdc42 GTPase-activating protein Bem3 from Saccharomyces cerevisiae (strain S288c) has been determined to a resolution of 2.2 A (Rwork = 21.1%, Rfree = 23.4%). It shows that the domains adopt a relative orientation that enables them to simultaneously bind to a membrane and suggests possible cooperativity in membrane binding.

Structure of the tandem PX-PH domains of Bem3 from Saccharomyces cerevisiae.,Ali I, Eu S, Koch D, Bleimling N, Goody RS, Muller MP Acta Crystallogr F Struct Biol Commun. 2018 May 1;74(Pt 5):315-321. doi:, 10.1107/S2053230X18005915. Epub 2018 Apr 24. PMID:29718000[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

  1. Zheng Y, Hart MJ, Shinjo K, Evans T, Bender A, Cerione RA. Biochemical comparisons of the Saccharomyces cerevisiae Bem2 and Bem3 proteins. Delineation of a limit Cdc42 GTPase-activating protein domain. J Biol Chem. 1993 Nov 25;268(33):24629-34. PMID:8227021
  2. Zheng Y, Cerione R, Bender A. Control of the yeast bud-site assembly GTPase Cdc42. Catalysis of guanine nucleotide exchange by Cdc24 and stimulation of GTPase activity by Bem3. J Biol Chem. 1994 Jan 28;269(4):2369-72. PMID:8300560
  3. Ali I, Eu S, Koch D, Bleimling N, Goody RS, Muller MP. Structure of the tandem PX-PH domains of Bem3 from Saccharomyces cerevisiae. Acta Crystallogr F Struct Biol Commun. 2018 May 1;74(Pt 5):315-321. doi:, 10.1107/S2053230X18005915. Epub 2018 Apr 24. PMID:29718000 doi:http://dx.doi.org/10.1107/S2053230X18005915

Contents


PDB ID 6fsf

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