Structural highlights
6gd8 is a 4 chain structure with sequence from Baker's yeast. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Ligands: | , |
| Related: | 6gd6, 6gd7, 6gd9, 6gda |
| Gene: | CYC1, YJR048W, J1653 (Baker's yeast) |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
[CYC1_YEAST] Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
Publication Abstract from PubMed
Controlled protein assembly provides a means to regulate function. Supramolecular building blocks, including rigid macrocycles, have proven to be versatile triggers of protein assembly. Here, we show that sulfonato-calix[8]arene (sclx8) mediates the formation of cytochrome c tetramers in solution. This tetramer spontaneously disassembles at >/= 2 equivalents of sclx8 providing a remarkable example of "auto-regulation". Using X-ray crystallography we characterize in detail the sclx8 binding sites on cytochrome c. Crystal structures at different protein-ligand ratios reveal varying degrees (up to ~35 %) of protein surface coverage by the calixarene and suggest a mechanism for oligomer disassembly. The solution structure of the oligomer was characterized by small angle X-ray scattering. Overall, the data indicate calixarene-controlled protein assembly and disassembly without the requirement for a competitive inhibitor, and point to protein encapsulation by a flexible macrocycle.
Auto-regulated Protein Assembly on a Supramolecular Scaffold.,Rennie M, Fox G, Perez J, Crowley PB Angew Chem Int Ed Engl. 2018 Aug 15. doi: 10.1002/anie.201807490. PMID:30109907[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Rennie M, Fox G, Perez J, Crowley PB. Auto-regulated Protein Assembly on a Supramolecular Scaffold. Angew Chem Int Ed Engl. 2018 Aug 15. doi: 10.1002/anie.201807490. PMID:30109907 doi:http://dx.doi.org/10.1002/anie.201807490
