6h6o
From Proteopedia
UbiJ-SCP2 Ubiquinone synthesis protein
Structural highlights
Function[UBIJ_ECOLI] Required for ubiquinone (coenzyme Q) biosynthesis under aerobic conditions.[1] Publication Abstract from PubMedUbiquinone (UQ) is a polyprenylated lipid that is conserved from bacteria to humans and is crucial to cellular respiration. How the cell orchestrates the efficient synthesis of UQ, which involves the modification of extremely hydrophobic substrates by multiple sequential enzymes, remains an unresolved issue. Here, we demonstrate that seven Ubi proteins form the Ubi complex, a stable metabolon that catalyzes the last six reactions of the UQ biosynthetic pathway in Escherichia coli. The SCP2 domain of UbiJ forms an extended hydrophobic cavity that binds UQ intermediates inside the 1-MDa Ubi complex. We purify the Ubi complex from cytoplasmic extracts and demonstrate that UQ biosynthesis occurs in this fraction, challenging the current thinking of a membrane-associated biosynthetic process. Collectively, our results document a rare case of stable metabolon and highlight how the supramolecular organization of soluble enzymes allows the modification of hydrophobic substrates in a hydrophilic environment. A Soluble Metabolon Synthesizes the Isoprenoid Lipid Ubiquinone.,Hajj Chehade M, Pelosi L, Fyfe CD, Loiseau L, Rascalou B, Brugiere S, Kazemzadeh K, Vo CD, Ciccone L, Aussel L, Coute Y, Fontecave M, Barras F, Lombard M, Pierrel F Cell Chem Biol. 2018 Dec 17. pii: S2451-9456(18)30439-2. doi:, 10.1016/j.chembiol.2018.12.001. PMID:30686758[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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