6i4n

From Proteopedia

Jump to: navigation, search

Dodecamer DNA containing the synthetic base pair P-Z in complex with a pyrrole-imidazole polyamide

Structural highlights

6i4n is a 2 chain structure. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:K4Z
NonStd Res:DP, DZ
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

The structural basis of minor groove recognition of a DNA duplex containing synthetic genetic information by hairpin pyrrole-imidazole polyamides is described. Hairpin polyamides induce a higher melting stabilization of a DNA duplex containing the unnatural P.Z base-pair when an imidazole unit is aligned with a P nucleotide. An NMR structural study showed that the incorporation of two isolated P.Z pairs enlarges the minor groove and slightly narrows the major groove at the site of this synthetic genetic information, relative to a DNA duplex consisting entirely of Watson-Crick base-pairs. Pyrrole-imidazole polyamides bind to a P.Z-containing DNA duplex to form a stable complex, effectively mimicking a G.C pair. A structural hallmark of minor groove recognition of a P.Z pair by a polyamide is the reduced level of allosteric distortion induced by binding of a polyamide to a DNA duplex. Understanding the molecular determinants that influence minor groove recognition of DNA containing synthetic genetic components provides the basis to further develop unnatural base-pairs for synthetic biology applications.

Sequence-Selective Minor Groove Recognition of a DNA Duplex Containing Synthetic Genetic Components.,Padroni G, Withers JM, Taladriz-Sender A, Reichenbach LF, Parkinson JA, Burley GA J Am Chem Soc. 2019 Jun 4. doi: 10.1021/jacs.8b12444. PMID:31117639[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

  1. Padroni G, Withers JM, Taladriz-Sender A, Reichenbach LF, Parkinson JA, Burley GA. Sequence-Selective Minor Groove Recognition of a DNA Duplex Containing Synthetic Genetic Components. J Am Chem Soc. 2019 Jun 4. doi: 10.1021/jacs.8b12444. PMID:31117639 doi:http://dx.doi.org/10.1021/jacs.8b12444

Contents


PDB ID 6i4n

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Personal tools