6o3t

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Structural basis of FOXC2 and DNA interactions

Structural highlights

6o3t is a 4 chain structure with sequence from Homo sapiens and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3.06Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

FOXC2_HUMAN Defects in FOXC2 are the cause of lymphedema hereditary type 2 (LMPH2) [MIM:153200; also known as Meige lymphedema. Hereditary lymphedema is a chronic disabling condition which results in swelling of the extremities due to altered lymphatic flow. Patients with lymphedema suffer from recurrent local infections, and physical impairment.[1] Defects in FOXC2 are a cause of lymphedema-yellow nails (LYYN) [MIM:153300. LYYN is characterized by yellow, dystrophic, thick and slowly growing nails, associated with lymphedema and respiratory involvement. Lymphedema occurs more often in the lower limbs. It can appear at birth or later in life. Onset generally follows the onset of ungual abnormalities. Defects in FOXC2 are a cause of lymphedema-distichiasis (LYD) [MIM:153400. LYD is characterized by primary limb lymphedema usually starting at puberty (but in some cases later or at birth) and associated with distichiasis (double rows of eyelashes, with extra eyelashes growing from the Meibomian gland orifices).[2]

Function

FOXC2_HUMAN Transcriptional activator. Might be involved in the formation of special mesenchymal tissues.[3]

Publication Abstract from PubMed

Forkhead transcription factor C2 (FOXC2) is a transcription factor regulating vascular and lymphatic development, and its mutations are linked to lymphedema-distichiasis syndrome. FOXC2 is also a crucial regulator of the epithelial-mesenchymal transition processes essential for tumor metastasis. Here, we report the crystal structure of the FOXC2-DNA-binding domain in complex with its cognate DNA. The crystal structure provides the basis of DNA sequence recognition by FOXC2 for the T/CAAAC motif. Helix 3 makes the majority of the DNA-protein interactions and confers the DNA sequence specificity. The computational energy calculation results also validate the structural observations. The FOXC2 and DNA complex structure provides a detailed picture of protein and DNA interactions, which allows us to predict its DNA recognition specificity and impaired functions in mutants identified in human patients.

Crystal Structure of FOXC2 in Complex with DNA Target.,Li S, Pradhan L, Ashur S, Joshi A, Nam HJ ACS Omega. 2019 Jun 24;4(6):10906-10914. doi: 10.1021/acsomega.9b00756. , eCollection 2019 Jun 30. PMID:31460188[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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See Also

References

  1. ↑ Fang J, Dagenais SL, Erickson RP, Arlt MF, Glynn MW, Gorski JL, Seaver LH, Glover TW. Mutations in FOXC2 (MFH-1), a forkhead family transcription factor, are responsible for the hereditary lymphedema-distichiasis syndrome. Am J Hum Genet. 2000 Dec;67(6):1382-8. Epub 2000 Nov 8. PMID:11078474 doi:S0002-9297(07)63207-8
  2. ↑ Bell R, Brice G, Child AH, Murday VA, Mansour S, Sandy CJ, Collin JR, Brady AF, Callen DF, Burnand K, Mortimer P, Jeffery S. Analysis of lymphoedema-distichiasis families for FOXC2 mutations reveals small insertions and deletions throughout the gene. Hum Genet. 2001 Jun;108(6):546-51. PMID:11499682
  3. ↑ Miura N, Iida K, Kakinuma H, Yang XL, Sugiyama T. Isolation of the mouse (MFH-1) and human (FKHL 14) mesenchyme fork head-1 genes reveals conservation of their gene and protein structures. Genomics. 1997 May 1;41(3):489-92. PMID:9169153 doi:S0888-7543(97)94695-4
  4. ↑ Li S, Pradhan L, Ashur S, Joshi A, Nam HJ. Crystal Structure of FOXC2 in Complex with DNA Target. ACS Omega. 2019 Jun 24;4(6):10906-10914. PMID:31460188 doi:10.1021/acsomega.9b00756

Contents


PDB ID 6o3t

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OCA

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