6oya

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Structure of the Rhodopsin-Transducin-Nanobody Complex

Structural highlights

6oya is a 5 chain structure with sequence from Bos taurus and Lama glama. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Electron Microscopy, Resolution 3.3Å
Experimental data:Check to display Experimental Data
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GNAT1_BOVIN Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase.

Publication Abstract from PubMed

Rhodopsin (Rho), a prototypical G-protein-coupled receptor (GPCR) in vertebrate vision, activates the G-protein transducin (GT) by catalyzing GDP-GTP exchange on its alpha subunit (GalphaT). To elucidate the determinants of GT coupling and activation, we obtained cryo-EM structures of a fully functional, light-activated Rho-GT complex in the presence and absence of a G-protein-stabilizing nanobody. The structures illustrate how GT overcomes its low basal activity by engaging activated Rho in a conformation distinct from other GPCR-G-protein complexes. Moreover, the nanobody-free structures reveal native conformations of G-protein components and capture three distinct conformers showing the GalphaT helical domain (alphaHD) contacting the Gbetagamma subunits. These findings uncover the molecular underpinnings of G-protein activation by visual rhodopsin and shed new light on the role played by Gbetagamma during receptor-catalyzed nucleotide exchange.

Structures of the Rhodopsin-Transducin Complex: Insights into G-Protein Activation.,Gao Y, Hu H, Ramachandran S, Erickson JW, Cerione RA, Skiniotis G Mol Cell. 2019 Jun 24. pii: S1097-2765(19)30440-X. doi:, 10.1016/j.molcel.2019.06.007. PMID:31300275[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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See Also

References

  1. Gao Y, Hu H, Ramachandran S, Erickson JW, Cerione RA, Skiniotis G. Structures of the Rhodopsin-Transducin Complex: Insights into G-Protein Activation. Mol Cell. 2019 Jun 24. pii: S1097-2765(19)30440-X. doi:, 10.1016/j.molcel.2019.06.007. PMID:31300275 doi:http://dx.doi.org/10.1016/j.molcel.2019.06.007

Contents


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6oya, resolution 3.30Å

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