6q9a

From Proteopedia

Structure of tmRNA SmpB bound past E site of E. coli 70S ribosome

Structural highlights

6q9a is a 10 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 3.7Å
Ligands:
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RS20_ECOLI Binds directly to 16S ribosomal RNA.[HAMAP-Rule:MF_00500]

Publication Abstract from PubMed

During trans-translation, transfer-messenger RNA (tmRNA) and small protein B (SmpB) together rescue ribosomes stalled on a truncated mRNA and tag the nascent polypeptide for degradation. We used cryo-electron microscopy to determine the structures of three key states of the tmRNA-SmpB-ribosome complex during trans translation at resolutions of 3.7 to 4.4 angstroms. The results show how tmRNA and SmpB act specifically on stalled ribosomes and how the circularized complex moves through the ribosome, enabling translation to switch from the old defective message to the reading frame on tmRNA.

How a circularized tmRNA moves through the ribosome.,Rae CD, Gordiyenko Y, Ramakrishnan V Science. 2019 Feb 15;363(6428):740-744. doi: 10.1126/science.aav9370. PMID:30765567^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations

reviews cite this structure

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References

↑ Rae CD, Gordiyenko Y, Ramakrishnan V. How a circularized tmRNA moves through the ribosome. Science. 2019 Feb 15;363(6428):740-744. doi: 10.1126/science.aav9370. PMID:30765567 doi:http://dx.doi.org/10.1126/science.aav9370