Structural highlights
6si7 is a 18 chain structure with sequence from "bacillus_coli"_migula_1895 "bacillus coli" migula 1895. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
|
Gene: | csgF, b1038, JW1021 ("Bacillus coli" Migula 1895), csgG, b1037, JW1020 ("Bacillus coli" Migula 1895) |
Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
[CSGG_ECOLI] May be involved in the biogenesis of curli organelles.
Publication Abstract from PubMed
Single-molecule long-read DNA sequencing with biological nanopores is fast and high-throughput but suffers reduced accuracy in homonucleotide stretches. We now combine the CsgG nanopore with the 35-residue N-terminal region of its extracellular interaction partner CsgF to produce a dual-constriction pore with improved signal and base-calling accuracy for homopolymer regions. The electron cryo-microscopy structure of CsgG in complex with full-length CsgF shows that the 33 N-terminal residues of CsgF bind inside the beta-barrel of the pore, forming a defined second constriction. In complexes of CsgG bound to a 35-residue CsgF constriction peptide, the second constriction is separated from the primary constriction by ~25 A. We find that both constrictions contribute to electrical signal modulation during single-stranded DNA translocation. DNA sequencing using a prototype CsgG-CsgF protein pore with two constrictions improved single-read accuracy by 25 to 70% in homopolymers up to 9 nucleotides long.
A dual-constriction biological nanopore resolves homonucleotide sequences with high fidelity.,Van der Verren SE, Van Gerven N, Jonckheere W, Hambley R, Singh P, Kilgour J, Jordan M, Wallace EJ, Jayasinghe L, Remaut H Nat Biotechnol. 2020 Jul 6. pii: 10.1038/s41587-020-0570-8. doi:, 10.1038/s41587-020-0570-8. PMID:32632300[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Van der Verren SE, Van Gerven N, Jonckheere W, Hambley R, Singh P, Kilgour J, Jordan M, Wallace EJ, Jayasinghe L, Remaut H. A dual-constriction biological nanopore resolves homonucleotide sequences with high fidelity. Nat Biotechnol. 2020 Jul 6. pii: 10.1038/s41587-020-0570-8. doi:, 10.1038/s41587-020-0570-8. PMID:32632300 doi:http://dx.doi.org/10.1038/s41587-020-0570-8