6tb3

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yeast 80S ribosome in complex with the Not5 subunit of the CCR4-NOT complex

Structural highlights

6tb3 is a 10 chain structure with sequence from Saccharomyces cerevisiae S288C. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Electron Microscopy, Resolution 2.8Å
Ligands:1MA, 1MG, 2MG, 5MC, 7MG, H2U, M2G, MG, SPD, T6A, ZN
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RSSA1_YEAST Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits.[1] [2]

Publication Abstract from PubMed

Control of messenger RNA (mRNA) decay rate is intimately connected to translation elongation, but the spatial coordination of these events is poorly understood. The Ccr4-Not complex initiates mRNA decay through deadenylation and activation of decapping. We used a combination of cryo-electron microscopy, ribosome profiling, and mRNA stability assays to examine the recruitment of Ccr4-Not to the ribosome via specific interaction of the Not5 subunit with the ribosomal E-site in Saccharomyces cerevisiae This interaction occurred when the ribosome lacked accommodated A-site transfer RNA, indicative of low codon optimality. Loss of the interaction resulted in the inability of the mRNA degradation machinery to sense codon optimality. Our findings elucidate a physical link between the Ccr4-Not complex and the ribosome and provide mechanistic insight into the coupling of decoding efficiency with mRNA stability.

The Ccr4-Not complex monitors the translating ribosome for codon optimality.,Buschauer R, Matsuo Y, Sugiyama T, Chen YH, Alhusaini N, Sweet T, Ikeuchi K, Cheng J, Matsuki Y, Nobuta R, Gilmozzi A, Berninghausen O, Tesina P, Becker T, Coller J, Inada T, Beckmann R Science. 2020 Apr 17;368(6488). pii: 368/6488/eaay6912. doi:, 10.1126/science.aay6912. PMID:32299921[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
23 reviews cite this structure
Roles of mRNA poly(A) tails in regulation of eukaryotic gene expression.
Passmore et al. (2022)
22 more
77 other articles
No citations found

See Also

References

  1. ↑ Ford CL, Randal-Whitis L, Ellis SR. Yeast proteins related to the p40/laminin receptor precursor are required for 20S ribosomal RNA processing and the maturation of 40S ribosomal subunits. Cancer Res. 1999 Feb 1;59(3):704-10. PMID:9973221
  2. ↑ Tabb-Massey A, Caffrey JM, Logsden P, Taylor S, Trent JO, Ellis SR. Ribosomal proteins Rps0 and Rps21 of Saccharomyces cerevisiae have overlapping functions in the maturation of the 3' end of 18S rRNA. Nucleic Acids Res. 2003 Dec 1;31(23):6798-805. PMID:14627813
  3. ↑ Buschauer R, Matsuo Y, Sugiyama T, Chen YH, Alhusaini N, Sweet T, Ikeuchi K, Cheng J, Matsuki Y, Nobuta R, Gilmozzi A, Berninghausen O, Tesina P, Becker T, Coller J, Inada T, Beckmann R. The Ccr4-Not complex monitors the translating ribosome for codon optimality. Science. 2020 Apr 17;368(6488). pii: 368/6488/eaay6912. doi:, 10.1126/science.aay6912. PMID:32299921 doi:http://dx.doi.org/10.1126/science.aay6912

Contents


PDB ID 6tb3

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