Structural highlights
Function
DABA_PSEMU Magnesium-dependent glutamate N-prenyltransferase: part of the gene cluster that mediates the biosynthesis of domoic acid (DA) and derivatives, natural products with neurochemical activity acting as ionotropic glutamate receptor (iGluR) agonists, thus being neurotoxins causing amnesic shellfish poisoning (ASP) (PubMed:30262498). Catalyzes the conversion of L-glutamic acid (L-Glu) to N-geranyl-L-glutamic acid (NGG) in the presence of geranyl diphosphate (GPP) (PubMed:30262498, PubMed:32457155). Also able to catalyze the formation of farnesyl-L-glutamate from farnesyl diphosphate (FPP) (PubMed:32457155). Cannot use dimethylallyl diphosphate (DMAPP) as substrate (PubMed:32457155).[1] [2]
References
- ↑ Brunson JK, McKinnie SMK, Chekan JR, McCrow JP, Miles ZD, Bertrand EM, Bielinski VA, Luhavaya H, Oborník M, Smith GJ, Hutchins DA, Allen AE, Moore BS. Biosynthesis of the neurotoxin domoic acid in a bloom-forming diatom. Science. 2018 Sep 28;361(6409):1356-1358. PMID:30262498 doi:10.1126/science.aau0382
- ↑ Chekan JR, McKinnie SMK, Noel JP, Moore BS. Algal neurotoxin biosynthesis repurposes the terpene cyclase structural fold into an N-prenyltransferase. Proc Natl Acad Sci U S A. 2020 May 26. pii: 2001325117. doi:, 10.1073/pnas.2001325117. PMID:32457155 doi:http://dx.doi.org/10.1073/pnas.2001325117
