6xy4 | pdb_00006xy4

Structural insight into sheep-pox virus mediated inhibition of apoptosis

spinqualitylabelsall models 6xy4, resolution 2.05Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6xy4 is a 2 chain structure with sequence from Homo sapiens and Sheeppox virus (strain TU-V02127). Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.0462315Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A3F2YKH3_SHEVT Publication Abstract from PubMed Programmed death of infected cells is used by multicellular organisms to counter viral infections. Sheeppox virus encodes for SPPV14, a potent inhibitor of Bcl-2-mediated apoptosis. We reveal the structural basis of apoptosis inhibition by determining crystal structures of SPPV14 bound to BH3 motifs of proapoptotic Bax and Hrk. The structures show that SPPV14 engages BH3 peptides using the canonical ligand-binding groove. Unexpectedly, Arg84 from SPPV14 forms an ionic interaction with the conserved Asp in the BH3 motif in a manner that replaces the canonical ionic interaction seen in almost all host Bcl-2:BH3 motif complexes. These results reveal the flexibility of virus-encoded Bcl-2 proteins to mimic key interactions from endogenous host signalling pathways to retain BH3 binding and prosurvival functionality. Crystal structures of the sheeppox virus encoded inhibitor of apoptosis SPPV14 bound to the proapoptotic BH3 peptides Hrk and Bax.,Suraweera CD, Burton DR, Hinds MG, Kvansakul M FEBS Lett. 2020 Jun;594(12):2016-2026. doi: 10.1002/1873-3468.13807. Epub 2020 , May 30. PMID:32390192 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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