6yef

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70S initiation complex with assigned rRNA modifications from Staphylococcus aureus

Structural highlights

6yef is a 10 chain structure with sequence from Staphylococcus aureus subsp. aureus NCTC 8325. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Electron Microscopy, Resolution 3.2Å
Ligands:2MA, 2MG, 31H, 4OC, 4SU, 5MU, 7MG, H2U, K, MA6, MG, OMC, OMG, PSU
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RS11_STAA8 Located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA. Forms part of the Shine-Dalgarno cleft in the 70S ribosome.

Publication Abstract from PubMed

Staphylococcus aureus is a bacterial pathogen and one of the leading causes of healthcare-acquired infections in the world. The growing antibiotic resistance of S. aureus obliges us to search for new drugs and treatments. As the majority of antibiotics target the ribosome, knowledge of its detailed structure is crucial for drug development. Here, we report the cryo-EM reconstruction at 3.2 A resolution of the S. aureus ribosome with P-site tRNA, messenger RNA, and 10 RNA modification sites previously not assigned or visualized. The resulting model is the most precise and complete high-resolution structure to date of the S. aureus 70S ribosome with functional ligands.

Cryo-EM structure of the ribosome functional complex of the human pathogen Staphylococcus aureus at 3.2 A resolution.,Golubev A, Fatkhullin B, Khusainov I, Jenner L, Gabdulkhakov A, Validov S, Yusupova G, Yusupov M, Usachev K FEBS Lett. 2020 Nov;594(21):3551-3567. doi: 10.1002/1873-3468.13915. Epub 2020 , Sep 10. PMID:32852796[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
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Structural basis of ABCF-mediated resistance to pleuromutilin, lincosamide, and streptogramin A antibiotics in Gram-positive pathogens.
Crowe-McAuliffe et al. (2021)
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5 other articles
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See Also

References

  1. ↑ Golubev A, Fatkhullin B, Khusainov I, Jenner L, Gabdulkhakov A, Validov S, Yusupova G, Yusupov M, Usachev K. Cryo-EM structure of the ribosome functional complex of the human pathogen Staphylococcus aureus at 3.2 Å resolution. FEBS Lett. 2020 Nov;594(21):3551-3567. PMID:32852796 doi:10.1002/1873-3468.13915

Contents


PDB ID 6yef

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