6zsq
From Proteopedia
Crystal structure of the Cisplatin beta-Lactoglobulin adduct formed after 18 h of soaking
Structural highlights
FunctionLACB_BOVIN Primary component of whey, it binds retinol and is probably involved in the transport of that molecule. Publication Abstract from PubMedbeta-Lactoglobulin is a major globular milk whey carrier with potential applications as an oral drug delivery system. Herein, the interactions between beta-lactoglobulin and cisplatin are investigated by UV-Vis absorption spectroscopy, circular dichroism, X-ray crystallography and electrospray ionization mass spectrometry. Structural data indicate that the protein retains its conformation upon cisplatin binding. Pt-containing fragments bind the side chains of Met7, His146 and Lys8, with the number of binding sites increasing over time. Mass spectrometry data indicate that [Pt(NH3)2Cl+], [Pt(NH3)2OH22+] and [Pt(NH3)22+] fragments interact with beta-lactoglobulin; up to 3 cisplatin fragments can bind the protein and the number of cisplatin binding sites increases over time. This work opens a new pathway in pharmaceutical studies based on a rational design of metal-based drug/beta-lactoglobulin adducts as delivering vehicles of metallodrugs. Cisplatin binding to beta-lactoglobulin: a structural study.,Balasco N, Ferraro G, Loreto D, Iacobucci I, Monti M, Merlino A Dalton Trans. 2020 Sep 15;49(35):12450-12457. doi: 10.1039/d0dt02582h. PMID:32852026[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
| ||||||||||||||||||||
