7acr

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Structure of post-translocated trans-translation complex on E. coli stalled ribosome.

Structural highlights

7acr is a 10 chain structure with sequence from Escherichia coli K-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Electron Microscopy, Resolution 3.44Å
Ligands:0TD, 1MG, 2MA, 2MG, 3TD, 4D4, 4OC, 5MC, 5MU, 6MZ, G7M, MA6, MG, OMC, OMG, OMU, PSU, UR3, ZN
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RL36_ECOLI

Publication Abstract from PubMed

In bacteria, trans-translation is the main rescue system, freeing ribosomes stalled on defective messenger RNAs. This mechanism is driven by small protein B (SmpB) and transfer-messenger RNA (tmRNA), a hybrid RNA known to have both a tRNA-like and an mRNA-like domain. Here we present four cryo-EM structures of the ribosome during trans-translation at resolutions from 3.0 to 3.4 A. These include the high-resolution structure of the whole pre-accommodated state, as well as structures of the accommodated state, the translocated state, and a translocation intermediate. Together, they shed light on the movements of the tmRNA-SmpB complex in the ribosome, from its delivery by the elongation factor EF-Tu to its passage through the ribosomal A and P sites after the opening of the B1 bridges. Additionally, we describe the interactions between the tmRNA-SmpB complex and the ribosome. These explain why the process does not interfere with canonical translation.

Structures of tmRNA and SmpB as they transit through the ribosome.,Guyomar C, D'Urso G, Chat S, Giudice E, Gillet R Nat Commun. 2021 Aug 13;12(1):4909. doi: 10.1038/s41467-021-24881-4. PMID:34389707[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
4 reviews cite this structure
<i>Trans</i>-Translation Is an Appealing Target for the Development of New Antimicrobial Compounds.
Campos-Silva et al. (2021)
3 more
13 other articles
No citations found

See Also

References

  1. ↑ Guyomar C, D'Urso G, Chat S, Giudice E, Gillet R. Structures of tmRNA and SmpB as they transit through the ribosome. Nat Commun. 2021 Aug 13;12(1):4909. PMID:34389707 doi:10.1038/s41467-021-24881-4

Contents


PDB ID 7acr

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OCA

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