7ax0

Publication Abstract from PubMed

Recent years have seen an increase in the development of computational proteins, including symmetric ones. A ninefold-symmetric beta-propeller protein named Cake has recently been developed. Here, attempts were made to further engineer this protein into a threefold-symmetric nine-bladed propeller using computational design. Two nine-bladed propeller proteins were designed, named Scone-E and Scone-R. Crystallography, however, revealed the structure of both designs to adopt an eightfold conformation with distorted termini, leading to a pseudo-symmetric protein. One of the proteins could only be crystallized upon the addition of a polyoxometalate, highlighting the usefulness of these molecules as crystallization additives.

Crystal structures of Scone: pseudosymmetric folding of a symmetric designer protein.,Mylemans B, Killian T, Vandebroek L, Van Meervelt L, Tame JRH, Parac-Vogt TN, Voet ARD Acta Crystallogr D Struct Biol. 2021 Jul 1;77(Pt 7):933-942. doi:, 10.1107/S2059798321005787. Epub 2021 Jun 18. PMID:34196619^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.