7b1z

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Virulence-associated protein VapB from the intracellular pathogen Rhodococcus equi

Structural highlights

7b1z is a 2 chain structure with sequence from Rhodococcus equi. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.71Å
Ligands:GOL, NO3
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

B4F366_RHOHA

Publication Abstract from PubMed

Virulence-associated proteins (Vaps) contribute to the virulence of the pathogen Rhodococcus equi, but their mode of action has remained elusive. All Vaps share a conserved core of about 105 amino acids that folds into a compact eight-stranded antiparallel beta-barrel with a unique topology. At the top of the barrel, four loops connect the eight beta-strands. Previous Vap structures did not show concave surfaces that might serve as a ligand-binding site. Here, the structure of VapB in a new crystal form was determined at 1.71 A resolution. The asymmetric unit contains two molecules. In one of them, the loop regions at the top of the barrel adopt a different conformation from other Vap structures. An outward movement of the loops results in the formation of a hydrophobic cavity that might act as a ligand-binding site. This lends further support to the hypothesis that the structural similarity between Vaps and avidins suggests a potential binding function for Vaps.

Conformational changes of loops highlight a potential binding site in Rhodococcus equi VapB.,Geerds C, Haas A, Niemann HH Acta Crystallogr F Struct Biol Commun. 2021 Aug 1;77(Pt 8):246-253. doi: , 10.1107/S2053230X2100738X. Epub 2021 Jul 28. PMID:34341190[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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References

  1. Geerds C, Haas A, Niemann HH. Conformational changes of loops highlight a potential binding site in Rhodococcus equi VapB. Acta Crystallogr F Struct Biol Commun. 2021 Aug 1;77(Pt 8):246-253. PMID:34341190 doi:10.1107/S2053230X2100738X

Contents


PDB ID 7b1z

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