7bms

Publication Abstract from PubMed

Over the last decades the phase problem in macromolecular x-ray crystallography has become more controllable as methods and approaches have diversified and improved. However, solving the phase problem is still one of the biggest obstacles on the way of successfully determining a crystal structure. To overcome this caveat, we have utilized the anomalous scattering properties of the heavy alkali metal cesium. We investigated the introduction of cesium in form of cesium chloride during the three major steps of protein treatment in crystallography: purification, crystallization, and cryo-protection. We derived a step-wise procedure encompassing a "quick-soak"-only approach and a combined approach of CsCl supplement during purification and cryo-protection. This procedure was successfully applied on two different proteins: (i) Lysozyme and (ii) as a proof of principle, a construct consisting of the PH domain of the TFIIH subunit p62 from Chaetomium thermophilum for de novo structure determination. Usage of CsCl thus provides a versatile, general, easy to use, and low cost phasing strategy.

Cesium based phasing of macromolecules: a general easy to use approach for solving the phase problem.,Koelmel W, Kuper J, Kisker C Sci Rep. 2021 Aug 23;11(1):17038. doi: 10.1038/s41598-021-95186-1. PMID:34426585^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.