| Structural highlights
Function
MYO17_ARATH Myosin heavy chain that is required for the cell cycle-regulated transport of various organelles and proteins for their segregation. Functions by binding with its tail domain to receptor proteins on organelles and exerting force with its N-terminal motor domain against actin filaments, thereby transporting its cargo along polarized actin cables. Involved in the tip growth of root hair cells and in the elongation of trichome stalk and branches. Plays a major role in trafficking of Golgi stacks, mitochondria and peroxisomes during root hair development. Acts as the primary contributor to ER streaming with a major role in the movement of Golgi bodies. Required for development of pavement cells, trichomes, and stigmatic papillae.[1] [2] [3] [4] [5] [6] [7] [8] [9]
Publication Abstract from PubMed
The plant-specific class XI myosins (MyoXIs) play key roles at the molecular, cellular and tissue levels, engaging diverse adaptor proteins to transport cargoes along actin filaments. To recognize their cargoes, MyoXIs have a C-terminal globular tail domain (GTD) that is evolutionarily related to those of class V myosins (MyoVs) from animals and fungi. Despite recent advances in understanding the functional roles played by MyoXI in plants, the structure of its GTD, and therefore the molecular determinants for cargo selectivity and recognition, remain elusive. In this study, the first crystal structure of a MyoXI GTD, that of MyoXI-K from Arabidopsis thaliana, was elucidated at 2.35 A resolution using a low-identity and fragment-based phasing approach in ARCIMBOLDO_SHREDDER. The results reveal that both the composition and the length of the alpha5-alpha6 loop are distinctive features of MyoXI-K, providing evidence for a structural stabilizing role for this loop, which is otherwise carried out by a molecular zipper in MyoV GTDs. The crystal structure also shows that most of the characterized cargo-binding sites in MyoVs are not conserved in plant MyoXIs, pointing to plant-specific cargo-recognition mechanisms. Notably, the main elements involved in the self-regulation mechanism of MyoVs are conserved in plant MyoXIs, indicating this to be an ancient ancestral trait.
Structure of the class XI myosin globular tail reveals evolutionary hallmarks for cargo recognition in plants.,Turowski VR, Ruiz DM, Nascimento AFZ, Millan C, Sammito MD, Juanhuix J, Cremonesi AS, Uson I, Giuseppe PO, Murakami MT Acta Crystallogr D Struct Biol. 2021 Apr 1;77(Pt 4):522-533. doi:, 10.1107/S2059798321001583. Epub 2021 Mar 30. PMID:33825712[10]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Ojangu EL, Järve K, Paves H, Truve E. Arabidopsis thaliana myosin XIK is involved in root hair as well as trichome morphogenesis on stems and leaves. Protoplasma. 2007;230(3-4):193-202. PMID:17458634 doi:10.1007/s00709-006-0233-8
- ↑ Peremyslov VV, Prokhnevsky AI, Avisar D, Dolja VV. Two class XI myosins function in organelle trafficking and root hair development in Arabidopsis. Plant Physiol. 2008 Mar;146(3):1109-16. doi: 10.1104/pp.107.113654. Epub 2008 Jan, 4. PMID:18178669 doi:http://dx.doi.org/10.1104/pp.107.113654
- ↑ Sparkes IA, Teanby NA, Hawes C. Truncated myosin XI tail fusions inhibit peroxisome, Golgi, and mitochondrial movement in tobacco leaf epidermal cells: a genetic tool for the next generation. J Exp Bot. 2008;59(9):2499-512. PMID:18503043 doi:10.1093/jxb/ern114
- ↑ Prokhnevsky AI, Peremyslov VV, Dolja VV. Overlapping functions of the four class XI myosins in Arabidopsis growth, root hair elongation, and organelle motility. Proc Natl Acad Sci U S A. 2008 Dec 16;105(50):19744-9. doi:, 10.1073/pnas.0810730105. Epub 2008 Dec 5. PMID:19060218 doi:http://dx.doi.org/10.1073/pnas.0810730105
- ↑ Avisar D, Abu-Abied M, Belausov E, Sadot E, Hawes C, Sparkes IA. A comparative study of the involvement of 17 Arabidopsis myosin family members on the motility of Golgi and other organelles. Plant Physiol. 2009 Jun;150(2):700-9. doi: 10.1104/pp.109.136853. Epub 2009 Apr, 15. PMID:19369591 doi:http://dx.doi.org/10.1104/pp.109.136853
- ↑ Ueda H, Yokota E, Kutsuna N, Shimada T, Tamura K, Shimmen T, Hasezawa S, Dolja VV, Hara-Nishimura I. Myosin-dependent endoplasmic reticulum motility and F-actin organization in plant cells. Proc Natl Acad Sci U S A. 2010 Apr 13;107(15):6894-9. PMID:20351265 doi:10.1073/pnas.0911482107
- ↑ Peremyslov VV, Prokhnevsky AI, Dolja VV. Class XI myosins are required for development, cell expansion, and F-Actin organization in Arabidopsis. Plant Cell. 2010 Jun;22(6):1883-97. doi: 10.1105/tpc.110.076315. Epub 2010 Jun, 25. PMID:20581304 doi:http://dx.doi.org/10.1105/tpc.110.076315
- ↑ Avisar D, Abu-Abied M, Belausov E, Sadot E. Myosin XIK is a major player in cytoplasm dynamics and is regulated by two amino acids in its tail. J Exp Bot. 2012 Jan;63(1):241-9. doi: 10.1093/jxb/err265. Epub 2011 Sep 13. PMID:21914656 doi:http://dx.doi.org/10.1093/jxb/err265
- ↑ Ojangu EL, Tanner K, Pata P, Jarve K, Holweg CL, Truve E, Paves H. Myosins XI-K, XI-1, and XI-2 are required for development of pavement cells, trichomes, and stigmatic papillae in Arabidopsis. BMC Plant Biol. 2012 Jun 6;12:81. doi: 10.1186/1471-2229-12-81. PMID:22672737 doi:http://dx.doi.org/10.1186/1471-2229-12-81
- ↑ Turowski VR, Ruiz DM, Nascimento AFZ, Millán C, Sammito MD, Juanhuix J, Cremonesi AS, Usón I, Giuseppe PO, Murakami MT. Structure of the class XI myosin globular tail reveals evolutionary hallmarks for cargo recognition in plants. Acta Crystallogr D Struct Biol. 2021 Apr 1;77(Pt 4):522-533. PMID:33825712 doi:10.1107/S2059798321001583
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