7lbf

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CryoEM structure of the HCMV Trimer gHgLgO in complex with human Platelet-derived growth factor receptor alpha and neutralizing fabs 13H11 and MSL-109

Structural highlights

7lbf is a 8 chain structure with sequence from Homo sapiens, Human betaherpesvirus 5 and Human herpesvirus 5 strain Merlin. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Electron Microscopy, Resolution 2.8Å
Ligands:BMA, MAN, NAG
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GH_HCMVM The heterodimer glycoprotein H-glycoprotein L is required for the fusion of viral and plasma membranes leading to virus entry into the host cell. Following initial binding to host receptor, membrane fusion is mediated by the fusion machinery composed of gB and the heterodimer gH/gL. May also be involved in the fusion between the virion envelope and the outer nuclear membrane during virion morphogenesis.

Publication Abstract from PubMed

Human cytomegalovirus (HCMV) infects the majority of the human population and represents the leading viral cause of congenital birth defects. HCMV utilizes the glycoproteins gHgLgO (Trimer) to bind to platelet-derived growth factor receptor alpha (PDGFRalpha) and transforming growth factor beta receptor 3 (TGFbetaR3) to gain entry into multiple cell types. This complex is targeted by potent neutralizing antibodies and represents an important candidate for therapeutics against HCMV. Here, we determine three cryogenic electron microscopy (cryo-EM) structures of the trimer and the details of its interactions with four binding partners: the receptor proteins PDGFRalpha and TGFbetaR3 as well as two broadly neutralizing antibodies. Trimer binding to PDGFRalpha and TGFbetaR3 is mutually exclusive, suggesting that they function as independent entry receptors. In addition, Trimer-PDGFRalpha interaction has an inhibitory effect on PDGFRalpha signaling. Our results provide a framework for understanding HCMV receptor engagement, neutralization, and the development of anti-viral strategies against HCMV.

Structures of HCMV Trimer reveal the basis for receptor recognition and cell entry.,Kschonsak M, Rouge L, Arthur CP, Hoangdung H, Patel N, Kim I, Johnson MC, Kraft E, Rohou AL, Gill A, Martinez-Martin N, Payandeh J, Ciferri C Cell. 2021 Mar 4;184(5):1232-1244.e16. doi: 10.1016/j.cell.2021.01.036. Epub 2021 , Feb 23. PMID:33626330[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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See Also

References

  1. ↑ Kschonsak M, Rouge L, Arthur CP, Hoangdung H, Patel N, Kim I, Johnson MC, Kraft E, Rohou AL, Gill A, Martinez-Martin N, Payandeh J, Ciferri C. Structures of HCMV Trimer reveal the basis for receptor recognition and cell entry. Cell. 2021 Mar 4;184(5):1232-1244.e16. doi: 10.1016/j.cell.2021.01.036. Epub 2021, Feb 23. PMID:33626330 doi:http://dx.doi.org/10.1016/j.cell.2021.01.036

Contents


PDB ID 7lbf

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