7lbm

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Structure of the human Mediator-bound transcription pre-initiation complex

Structural highlights

7lbm is a 10 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Electron Microscopy, Resolution 4.8Å
Ligands:MG, ZN
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MED16_HUMAN Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.[1] [2]

Publication Abstract from PubMed

Eukaryotic transcription requires the assembly of a multisubunit preinitiation complex (PIC) composed of RNA polymerase II (Pol II) and the general transcription factors. The coactivator Mediator is recruited by transcription factors, facilitates the assembly of the PIC, and stimulates phosphorylation of the Pol II C-terminal domain (CTD) by the TFIIH subunit CDK7. Here, we present the cryo-electron microscopy structure of the human Mediator-bound PIC at a resolution below 4 angstroms. Transcription factor binding sites within Mediator are primarily flexibly tethered to the tail module. CDK7 is stabilized by multiple contacts with Mediator. Two binding sites exist for the Pol II CTD, one between the head and middle modules of Mediator and the other in the active site of CDK7, providing structural evidence for Pol II CTD phosphorylation within the Mediator-bound PIC.

Structure of the human Mediator-bound transcription preinitiation complex.,Abdella R, Talyzina A, Chen S, Inouye CJ, Tjian R, He Y Science. 2021 Apr 2;372(6537):52-56. doi: 10.1126/science.abg3074. Epub 2021 Mar , 11. PMID:33707221[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
17 reviews cite this structure
The Mediator complex as a master regulator of transcription by RNA polymerase II.
Richter et al. (2022)
16 more
40 other articles
No citations found

See Also

References

  1. ↑ Ito M, Yuan CX, Malik S, Gu W, Fondell JD, Yamamura S, Fu ZY, Zhang X, Qin J, Roeder RG. Identity between TRAP and SMCC complexes indicates novel pathways for the function of nuclear receptors and diverse mammalian activators. Mol Cell. 1999 Mar;3(3):361-70. PMID:10198638 doi:10.1016/s1097-2765(00)80463-3
  2. ↑ Rachez C, Lemon BD, Suldan Z, Bromleigh V, Gamble M, Näär AM, Erdjument-Bromage H, Tempst P, Freedman LP. Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex. Nature. 1999 Apr 29;398(6730):824-8. PMID:10235266 doi:10.1038/19783
  3. ↑ Abdella R, Talyzina A, Chen S, Inouye CJ, Tjian R, He Y. Structure of the human Mediator-bound transcription preinitiation complex. Science. 2021 Apr 2;372(6537):52-56. PMID:33707221 doi:10.1126/science.abg3074

Contents


PDB ID 7lbm

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