7oh3

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Nog1-TAP associated immature ribosomal particle population B from S. cerevisiae

Structural highlights

7oh3 is a 10 chain structure with sequence from Saccharomyces cerevisiae S288C. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Electron Microscopy, Resolution 3.4Å
Ligands:MG, ZN
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CGR1_YEAST Involved in nucleolar integrity and required for processing of the pre-rRNA for the 60S ribosome subunit.[1] [2] [3]

Publication Abstract from PubMed

In yeast and human cells many of the ribosomal proteins (r-proteins) are required for the stabilisation and productive processing of rRNA precursors. Functional coupling of r-protein assembly with the stabilisation and maturation of subunit precursors potentially promotes the production of ribosomes with defined composition. To further decipher mechanisms of such an intrinsic quality control pathway we analysed here the contribution of three yeast large ribosomal subunit r-proteins rpL2 (uL2), rpL25 (uL23) and rpL34 (eL34) for intermediate nuclear subunit folding steps. Structure models obtained from single particle cryo-electron microscopy analyses provided evidence for specific and hierarchic effects on the stable positioning and remodelling of large ribosomal subunit domains. Based on these structural and previous biochemical data we discuss possible mechanisms of r-protein dependent hierarchic domain arrangement and the resulting impact on the stability of misassembled subunits.

Analysis of subunit folding contribution of three yeast large ribosomal subunit proteins required for stabilisation and processing of intermediate nuclear rRNA precursors.,Poll G, Pilsl M, Griesenbeck J, Tschochner H, Milkereit P PLoS One. 2021 Nov 23;16(11):e0252497. doi: 10.1371/journal.pone.0252497. , eCollection 2021. PMID:34813592[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
1 reviews cite this structure
Ribosome biogenesis factors-from names to functions.
Dörner et al. (2023)
0 more
2 other articles
No citations found

See Also

References

  1. ↑ Sun J, McFarland M, Boettner D, Panepinto J, Rhodes JC, Askew DS. Cgr1p, a novel nucleolar protein encoded by Saccharomyces cerevisiae orf YGL0292w. Curr Microbiol. 2001 Jan;42(1):65-9. PMID:11116400
  2. ↑ Moy TI, Boettner D, Rhodes JC, Silver PA, Askew DS. Identification of a role for Saccharomyces cerevisiae Cgr1p in pre-rRNA processing and 60S ribosome subunit synthesis. Microbiology. 2002 Apr;148(Pt 4):1081-90. PMID:11932453
  3. ↑ Wade CH, Umbarger MA, McAlear MA. The budding yeast rRNA and ribosome biosynthesis (RRB) regulon contains over 200 genes. Yeast. 2006 Mar;23(4):293-306. PMID:16544271 doi:http://dx.doi.org/10.1002/yea.1353
  4. ↑ Pöll G, Pilsl M, Griesenbeck J, Tschochner H, Milkereit P. Analysis of subunit folding contribution of three yeast large ribosomal subunit proteins required for stabilisation and processing of intermediate nuclear rRNA precursors. PLoS One. 2021 Nov 23;16(11):e0252497. PMID:34813592 doi:10.1371/journal.pone.0252497

Contents


PDB ID 7oh3

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OCA

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