7pob

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An Irreversible, Promiscuous and Highly Thermostable Claisen-Condensation Biocatalyst Drives the Synthesis of Substituted Pyrroles

Structural highlights

7pob is a 4 chain structure with sequence from Thermus thermophilus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2Å
Ligands:NA, PLP
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

BIKB_THET8 Catalyzes the decarboxylative condensation of pimeloyl-[acyl-carrier protein] and L-alanine to produce 8-amino-7-oxononanoate (AON), [acyl-carrier protein], and carbon dioxide (By similarity). Can also use pimeloyl-CoA instead of pimeloyl-ACP as substrate. It also converts 2-amino-3-ketobutyrate and CoA to glycine and acetyl-CoA. Activity is also observed with the following combinations of substrates: acetyl-CoA and either L-alanine or L-serine, pimeloyl-CoA and either glycine or L-serine, and palmitoyl-CoA with L-alanine.[1]

References

  1. ↑ Kubota T, Shimono J, Kanameda C, Izumi Y. The first thermophilic alpha-oxoamine synthase family enzyme that has activities of 2-amino-3-ketobutyrate CoA ligase and 7-keto-8-aminopelargonic acid synthase: cloning and overexpression of the gene from an extreme thermophile, Thermus thermophilus, and characterization of its gene product. Biosci Biotechnol Biochem. 2007 Dec;71(12):3033-40. doi: 10.1271/bbb.70438. Epub , 2007 Dec 7. PMID:18071260 doi:http://dx.doi.org/10.1271/bbb.70438

Contents


PDB ID 7pob

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OCA

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