7r6q

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State E2 nucleolar 60S ribosome biogenesis intermediate - Foot region model

Structural highlights

7r6q is a 10 chain structure with sequence from Saccharomyces cerevisiae BY4741. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Electron Microscopy, Resolution 2.98Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

HAS1_YEAST ATP-dependent RNA helicase involved in 40S ribosomal subunit biogenesis. Required for the processing and cleavage of 35S pre-rRNA at sites A0, A1, and A2, leading to mature 18S rRNA.[1] [2] [3]

Publication Abstract from PubMed

DEAD-box ATPases are ubiquitous enzymes essential in all aspects of RNA biology. However, the limited in vitro catalytic activities described for these enzymes are at odds with their complex cellular roles, most notably in driving large-scale RNA remodeling steps during the assembly of ribonucleoproteins (RNPs). We describe cryo-EM structures of 60S ribosomal biogenesis intermediates that reveal how context-specific RNA unwinding by the DEAD-box ATPase Spb4 results in extensive, sequence-specific remodeling of rRNA secondary structure. Multiple cis and trans interactions stabilize Spb4 in a post-catalytic, high-energy intermediate that drives the organization of the three-way junction at the base of rRNA domain IV. This mechanism explains how limited strand separation by DEAD-box ATPases is leveraged to provide non-equilibrium directionality and ensure efficient and accurate RNP assembly.

Sequence-specific remodeling of a topologically complex RNP substrate by Spb4.,Cruz VE, Sekulski K, Peddada N, Sailer C, Balasubramanian S, Weirich CS, Stengel F, Erzberger JP Nat Struct Mol Biol. 2022 Dec;29(12):1228-1238. doi: 10.1038/s41594-022-00874-9. , Epub 2022 Dec 8. PMID:36482249[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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See Also

References

  1. ↑ Emery B, de la Cruz J, Rocak S, Deloche O, Linder P. Has1p, a member of the DEAD-box family, is required for 40S ribosomal subunit biogenesis in Saccharomyces cerevisiae. Mol Microbiol. 2004 Apr;52(1):141-58. doi: 10.1111/j.1365-2958.2003.03973.x. PMID:15049817 doi:http://dx.doi.org/10.1111/j.1365-2958.2003.03973.x
  2. ↑ Mnaimneh S, Davierwala AP, Haynes J, Moffat J, Peng WT, Zhang W, Yang X, Pootoolal J, Chua G, Lopez A, Trochesset M, Morse D, Krogan NJ, Hiley SL, Li Z, Morris Q, Grigull J, Mitsakakis N, Roberts CJ, Greenblatt JF, Boone C, Kaiser CA, Andrews BJ, Hughes TR. Exploration of essential gene functions via titratable promoter alleles. Cell. 2004 Jul 9;118(1):31-44. PMID:15242642 doi:10.1016/j.cell.2004.06.013
  3. ↑ Rocak S, Emery B, Tanner NK, Linder P. Characterization of the ATPase and unwinding activities of the yeast DEAD-box protein Has1p and the analysis of the roles of the conserved motifs. Nucleic Acids Res. 2005 Feb 17;33(3):999-1009. doi: 10.1093/nar/gki244. Print, 2005. PMID:15718299 doi:http://dx.doi.org/10.1093/nar/gki244
  4. ↑ Cruz VE, Sekulski K, Peddada N, Sailer C, Balasubramanian S, Weirich CS, Stengel F, Erzberger JP. Sequence-specific remodeling of a topologically complex RNP substrate by Spb4. Nat Struct Mol Biol. 2022 Dec;29(12):1228-1238. PMID:36482249 doi:10.1038/s41594-022-00874-9

Contents


PDB ID 7r6q

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OCA

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