7sva

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Cryo-EM structure of Arabidopsis Ago10-guide RNA complex

Structural highlights

7sva is a 2 chain structure with sequence from Arabidopsis thaliana and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Electron Microscopy, Resolution 3.26Å
Ligands:MG
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

AGO10_ARATH Involved in RNA-mediated post-transcriptional gene silencing (PTGS). Main component of the RNA-induced silencing complex (RISC) that binds to a short guide RNA such as a microRNA (miRNA) or small interfering RNA (siRNA). RISC uses the mature miRNA or siRNA as a guide for slicer-directed cleavage of homologous mRNAs to repress gene expression. Required for reliable formation of primary and axillary shoot apical meristems. Specifies leaf adaxial identity by repressing the miR165 and miR166 microRNAs in the embryonic shoot apex, in the shoot apical meristem (SAM) and leaf. Represses the microRNA miR398 which targets CCS1 chaperone mRNAs for translational inhibition. Acts as a negative regulator of AGO1 protein level. Like AGO1, is required for stem cell function and organ polarity. Unlike AGO1, is not subjected to small RNA-mediated repression itself. Essential for multiple processes in development. Coregulates, with GATA18/HAN, the shoot apical meristem (SAM) organization (PubMed:26390296).[1] [2] [3] [4] [5] [6] [7]

Publication Abstract from PubMed

Argonaute (AGO) proteins use small RNAs to recognize transcripts targeted for silencing in plants and animals. Many AGOs cleave target RNAs using an endoribonuclease activity termed 'slicing'. Slicing by DNA-guided prokaryotic AGOs has been studied in detail, but structural insights into RNA-guided slicing by eukaryotic AGOs are lacking. Here we present cryogenic electron microscopy structures of the Arabidopsis thaliana Argonaute10 (AtAgo10)-guide RNA complex with and without a target RNA representing a slicing substrate. The AtAgo10-guide-target complex adopts slicing-competent and slicing-incompetent conformations that are unlike known prokaryotic AGO structures. AtAgo10 slicing activity is licensed by docking target (t) nucleotides t9-t13 into a surface channel containing the AGO endoribonuclease active site. A beta-hairpin in the L1 domain secures the t9-t13 segment and coordinates t9-t13 docking with extended guide-target pairing. Results show that prokaryotic and eukaryotic AGOs use distinct mechanisms for achieving target slicing and provide insights into small interfering RNA potency.

Structural basis for RNA slicing by a plant Argonaute.,Xiao Y, Maeda S, Otomo T, MacRae IJ Nat Struct Mol Biol. 2023 Jun;30(6):778-784. doi: 10.1038/s41594-023-00989-7. , Epub 2023 May 1. PMID:37127820[8]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
2 reviews cite this structure
When Argonaute takes out the ribonuclease sword.
Nakanishi et al. (2024)
1 more
3 other articles
No citations found

References

  1. ↑ Newman KL, Fernandez AG, Barton MK. Regulation of axis determinacy by the Arabidopsis PINHEAD gene. Plant Cell. 2002 Dec;14(12):3029-42. doi: 10.1105/tpc.005132. PMID:12468725 doi:http://dx.doi.org/10.1105/tpc.005132
  2. ↑ Liu Q, Yao X, Pi L, Wang H, Cui X, Huang H. The ARGONAUTE10 gene modulates shoot apical meristem maintenance and establishment of leaf polarity by repressing miR165/166 in Arabidopsis. Plant J. 2009 Apr;58(1):27-40. doi: 10.1111/j.1365-313X.2008.03757.x. Epub 2008, Dec 29. PMID:19054365 doi:http://dx.doi.org/10.1111/j.1365-313X.2008.03757.x
  3. ↑ Mallory AC, Hinze A, Tucker MR, Bouche N, Gasciolli V, Elmayan T, Lauressergues D, Jauvion V, Vaucheret H, Laux T. Redundant and specific roles of the ARGONAUTE proteins AGO1 and ZLL in development and small RNA-directed gene silencing. PLoS Genet. 2009 Sep;5(9):e1000646. doi: 10.1371/journal.pgen.1000646. Epub 2009 , Sep 18. PMID:19763164 doi:http://dx.doi.org/10.1371/journal.pgen.1000646
  4. ↑ Beauclair L, Yu A, Bouche N. microRNA-directed cleavage and translational repression of the copper chaperone for superoxide dismutase mRNA in Arabidopsis. Plant J. 2010 May;62(3):454-62. doi: 10.1111/j.1365-313X.2010.04162.x. Epub 2010 , Feb 1. PMID:20128885 doi:http://dx.doi.org/10.1111/j.1365-313X.2010.04162.x
  5. ↑ Ding L, Yan S, Jiang L, Zhao W, Ning K, Zhao J, Liu X, Zhang J, Wang Q, Zhang X. HANABA TARANU (HAN) Bridges Meristem and Organ Primordia Boundaries through PINHEAD, JAGGED, BLADE-ON-PETIOLE2 and CYTOKININ OXIDASE 3 during Flower Development in Arabidopsis. PLoS Genet. 2015 Sep 21;11(9):e1005479. doi: 10.1371/journal.pgen.1005479., eCollection 2015 Sep. PMID:26390296 doi:http://dx.doi.org/10.1371/journal.pgen.1005479
  6. ↑ Moussian B, Schoof H, Haecker A, Jurgens G, Laux T. Role of the ZWILLE gene in the regulation of central shoot meristem cell fate during Arabidopsis embryogenesis. EMBO J. 1998 Mar 16;17(6):1799-809. doi: 10.1093/emboj/17.6.1799. PMID:9501101 doi:http://dx.doi.org/10.1093/emboj/17.6.1799
  7. ↑ Lynn K, Fernandez A, Aida M, Sedbrook J, Tasaka M, Masson P, Barton MK. The PINHEAD/ZWILLE gene acts pleiotropically in Arabidopsis development and has overlapping functions with the ARGONAUTE1 gene. Development. 1999 Feb;126(3):469-81. PMID:9876176
  8. ↑ Xiao Y, Maeda S, Otomo T, MacRae IJ. Structural basis for RNA slicing by a plant Argonaute. Nat Struct Mol Biol. 2023 Jun;30(6):778-784. PMID:37127820 doi:10.1038/s41594-023-00989-7

Contents


PDB ID 7sva

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