7uim
From Proteopedia
CryoEM Structure of an Group II Intron Retroelement (apo-complex)
Structural highlights
FunctionPublication Abstract from PubMedGroup II introns are ribozymes that catalyze their self-excision and function as retroelements that invade DNA. As retrotransposons, group II introns form ribonucleoprotein (RNP) complexes that roam the genome, integrating by reversal of forward splicing. Here we show that retrotransposition is achieved by a tertiary complex between a structurally elaborate ribozyme, its protein mobility factor, and a structured DNA substrate. We solved cryo-electron microscopy structures of an intact group IIC intron-maturase retroelement that was poised for integration into a DNA stem-loop motif. By visualizing the RNP before and after DNA targeting, we show that it is primed for attack and fits perfectly with its DNA target. This study reveals design principles of a prototypical retroelement and reinforces the hypothesis that group II introns are ancient elements of genetic diversification. Structures of a mobile intron retroelement poised to attack its structured DNA target.,Chung K, Xu L, Chai P, Peng J, Devarkar SC, Pyle AM Science. 2022 Nov 11;378(6620):627-634. doi: 10.1126/science.abq2844. Epub 2022 , Nov 10. PMID:36356138[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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