7vso

From Proteopedia

Serial Femtosecond Crystallography (SFX) of Ground State Bacteriorhodopsin Crystallized from Bicelles in Complex with HAD16 Determined Using 7-keV X-ray Free Electron Laser (XFEL) at SACLA

Structural highlights

7vso is a 1 chain structure with sequence from Halobacterium salinarum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.35Å
Ligands:	7YH, CPS, D10, D12, DD9, HP6, IOD, OCT, R16, RET
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

BACR_HALSA Light-driven proton pump.

Publication Abstract from PubMed

Membrane proteins reside in the lipid bilayer of biomembranes and the structure and function of these proteins are closely related to their interactions with lipid molecules. Structural analyses of interactions between membrane proteins and lipids or detergents that constitute biological or artificial model membranes are important for understanding the functions and physicochemical properties of membrane proteins and biomembranes. Determination of membrane protein structures is much more difficult when compared with that of soluble proteins, but the development of various new technologies has accelerated the elucidation of the structure-function relationship of membrane proteins. This review summarizes the development of heavy atom derivative detergents and lipids that can be used for structural analysis of membrane proteins and their interactions with detergents/lipids, including their application with X-ray free-electron laser crystallography.

Heavy Atom Detergent/Lipid Combined X-ray Crystallography for Elucidating the Structure-Function Relationships of Membrane Proteins.,Hanashima S, Nakane T, Mizohata E Membranes (Basel). 2021 Oct 27;11(11). pii: membranes11110823. doi:, 10.3390/membranes11110823. PMID:34832053^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations

2 reviews cite this structure

Protein Lipidation Types: Current Strategies for Enrichment and Characterization.

Wang et al. (2022)

1 more

1 other articles

No citations found

References

↑ Hanashima S, Nakane T, Mizohata E. Heavy Atom Detergent/Lipid Combined X-ray Crystallography for Elucidating the Structure-Function Relationships of Membrane Proteins. Membranes (Basel). 2021 Oct 27;11(11):823. PMID:34832053 doi:10.3390/membranes11110823

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

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PDB ID 7vso

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Categories: Halobacterium salinarum | Large Structures | Hanashima S | Mizohata E | Nakane T

7vso

From Proteopedia

Serial Femtosecond Crystallography (SFX) of Ground State Bacteriorhodopsin Crystallized from Bicelles in Complex with HAD16 Determined Using 7-keV X-ray Free Electron Laser (XFEL) at SACLA

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

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